ArticlePloS one2012
Grapevine aquaporins: gating of a tonoplast intrinsic protein (TIP2;1) by cytosolic pH.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 74 citations in OpenAlex.
- Cork oak aquaporins: functional diversity and regulation with insights into drought response.Plant cell reports · 2026Article
- A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels.Current protocols · 2026Article
- Transcriptional Analysis Reveals the Differences in Response of Floral Buds to Boron Deficiency Between Two ContrastingPlants (Basel, Switzerland) · 2025Article
- Analysis of sulfide signaling in rice highlights specific drought responses.Journal of experimental botany · 2024Article
- TIP aquaporins in Cyperus esculentus: genome-wide identification, expression profiles, subcellular localizations, and interaction patterns.BMC plant biology · 2024Article
- Biochemical Response and Gene Expression to Water Deficit of Croatian Grapevine Cultivars (Plants (Basel, Switzerland) · 2023Article
- Aquaporin Gating: A New Twist to Unravel Permeation through Water Channels.International journal of molecular sciences · 2022Review
- Effectors of Root-Knot Nematodes: An Arsenal for Successful Parasitism.Frontiers in plant science · 2021Review
- Insights into the Selectivity Mechanisms of Grapevine NIP Aquaporins.International journal of molecular sciences · 2020Article
- Genome-wide identification and characterisation of Aquaporins in Nicotiana tabacum and their relationships with other Solanaceae species.BMC plant biology · 2020Article
- Sweet Cherry (International journal of molecular sciences · 2020Article
- Water Deficit Affects the Growth and Leaf Metabolite Composition of Young Loquat Plants.Plants (Basel, Switzerland) · 2020Article
- Molecular and Functional Characterization of Grapevine NIPs through Heterologous Expression inInternational journal of molecular sciences · 2020Article
- Dynamic TMT-Based Quantitative Proteomics Analysis of Critical Initiation Process of Totipotency during Cotton Somatic Embryogenesis Transdifferentiation.International journal of molecular sciences · 2019Article
- Article
- Human adipose glycerol flux is regulated by a pH gate in AQP10.Nature communications · 2018Article
- Review
- Article
- Permeability and ammonia selectivity in aquaporin TIP2;1: linking structure to function.Scientific reports · 2018Article
- Plant and animal aquaporins crosstalk: what can be revealed from distinct perspectives.Biophysical reviews · 2017Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Grapevine (Vitis vinifera L.) is one of the oldest and most important perennial crops being considered as a fruit ligneous tree model system in which the water status appears crucial for high fruit and wine quality, controlling productivity and alcohol level. V. vinifera genome contains 28 genes coding for aquaporins, which acting in a concerted and regulated manner appear relevant for plant withstanding extremely unfavorable drought conditions essential for the quality of berries and wine. Several Vv aquaporins have been reported to be expressed in roots, shoots, berries and leaves with clear cultivar differences in their expression level, making their in vivo biochemical characterization a difficult task. In this work V. vinifera cv. Touriga nacional VvTnPIP1;1, VvTnPIP2;2 and VvTnTIP2;1 were expressed in yeast and water transport activity was characterized in intact cells of the transformants. The three aquaporins were localized in the yeast plasma membrane but only VvTnTIP2;1 expression enhanced the water permeability with a concomitant decrease of the activation energy of water transport. Acidification of yeast cytosol resulted in loss of VvTnTIP2;1 activity. Sequence analysis revealed the presence of a His(131) residue, unusual in TIPs. By site directed mutagenesis, replacement of this residue by aspartic acid or alanine resulted in loss of pH(in) dependence while replacement by lysine resulted in total loss of activity. In addition to characterization of VvTn aquaporins, these results shed light on the gating of a specific tonoplast aquaporin by cytosolic pH.
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