ArticlePloS one2012
Electron tomography of fusiform vesicles and their organization in urothelial cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- The urothelial gene regulatory network: understanding biology to improve bladder cancer management.Oncogene · 2024Review
- Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated Manner.Metabolites · 2022Article
- Engineered human organ-specific urethra as a functional substitute.Scientific reports · 2022Article
- Cryo-EM structures of peripherin-2 and ROM1 suggest multiple roles in photoreceptor membrane morphogenesis.Science advances · 2022Article
- The Golgi complex: An organelle that determines urothelial cell biology in health and disease.Histochemistry and cell biology · 2022Review
- Segmentation of the urothelium in optical coherence tomography images with dynamic contrast.Journal of biomedical optics · 2021Article
- The MAL Protein, an Integral Component of Specialized Membranes, in Normal Cells and Cancer.Cells · 2021Review
- Immunohistochemistry as a paramount tool in research of normal urothelium, bladder cancer and bladder pain syndrome.European journal of histochemistry : EJH · 2021Review
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Extracellular Vesicles Derived Human-miRNAs Modulate the Immune System in Type 1 Diabetes.Frontiers in cell and developmental biology · 2020Article
- Electron tomography-a tool for ultrastructural 3D visualization in cell biology and histology.Wiener medizinische Wochenschrift (1946) · 2018Review
- Uroplakin traffic through the Golgi apparatus induces its fragmentation: new insights from novel in vitro models.Scientific reports · 2017Article
- Sequential and compartmentalized action of Rabs, SNAREs, and MAL in the apical delivery of fusiform vesicles in urothelial umbrella cells.Molecular biology of the cell · 2016Article
- Cystitis: from urothelial cell biology to clinical applications.BioMed research international · 2014Review
- Article
- A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells.Molecular biology of the cell · 2013Article
- Heterogeneity of uroplakin localization in human normal urothelium, papilloma and papillary carcinoma.Radiology and oncology · 2013Article
- Gold nanoparticles as physiological markers of urine internalization into urothelial cells in vivo.International journal of nanomedicine · 2013Article
- Trefoil Factor Protein 3 (TFF3) as a Guardian of the Urinary Bladder Epithelium.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of fusiform vesicles (FVs) is one of the most distinctive features in the urothelium of the urinary bladder. FVs represent compartments for intracellular transport of urothelial plaques, which modulate the surface area of the superficial urothelial (umbrella) cells during the distension-contraction cycle. We have analysed the three-dimensional (3D) structure of FVs and their organization in umbrella cells of mouse urinary bladders. Compared to chemical fixation, high pressure freezing gave a new insight into the ultrastructure of urothelial cells. Electron tomography on serial sections revealed that mature FVs had a shape of flattened discs, with a diameter of up to 1.2 µm. The lumen between the two opposing asymmetrically thickened membranes was very narrow, ranging from 5 nm to 10 nm. Freeze-fracturing and immunolabelling confirmed that FVs contain two opposing urothelial plaques connected by a hinge region that made an omega shaped curvature. In the central cytoplasm, 4-15 FVs were often organized into stacks. In the subapical cytoplasm, FVs were mainly organized as individual vesicles. Distension-contraction cycles did not affect the shape of mature FVs; however, their orientation changed from parallel in distended to perpendicular in contracted bladder with respect to the apical plasma membrane. In the intermediate cells, shorter and more dilated immature FVs were present. The salient outcome from this research is the first comprehensive, high resolution 3D view of the ultrastructure of FVs and how they are organized differently depending on their location in the cytoplasm of umbrella cells. The shape of mature FVs and their organization into tightly packed stacks makes them a perfect storage compartment, which transports large amounts of urothelial plaques while occupying a small volume of umbrella cell cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.