ArticlePloS one2012
Conversion of t11t13 CLA into c9t11 CLA in Caco-2 cells and inhibition by sterculic oil.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03612700 (Presence of Cyclopropane Fatty Acids), which is not on this map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Presence of Cyclopropane Fatty Acids (CPFA) in Human Plasma After CPFA-rich Diet
Who cites it
9 citing papers in PubMed, 34 citations in OpenAlex.
- Identification of Cyclopropane Fatty Acids in Human Plasma after Controlled Dietary Intake of Specific Foods.Nutrients · 2020Trial
- Punicic Acid Triggers Ferroptotic Cell Death in Carcinoma Cells.Nutrients · 2021Article
- Modulations of lipid metabolism and development of the Atlantic salmon (Salmo salar) fry in response to egg-to-fry rearing conditions.Fish physiology and biochemistry · 2021Article
- Determination of Cyclopropenoid Fatty Acids in Ewe Milk Fat by GC-MS after Intravenous Administration of Sterculic Acid.Foods (Basel, Switzerland) · 2020Article
- Interplay Between Plasma Membrane Lipid Alteration, Oxidative Stress and Calcium-Based Mechanism for Extracellular Vesicle Biogenesis From Erythrocytes During Blood Storage.Frontiers in physiology · 2020Article
- Sequential Feeding of Lipid Supplement Enriches Beef Adipose Tissues with 18:3n-3 Biohydrogenation Intermediates.Lipids · 2017Article
- Efficient in vitro adipocyte model of long-term lipolysis: a tool to study the behavior of lipophilic compounds.In vitro cellular & developmental biology. Animal · 2014Article
- Polyunsaturated fatty acid metabolism in enterocyte models: T84 cell line vs. Caco-2 cell line.In vitro cellular & developmental biology. Animal · 2014Article
- Trans-vaccenate is Δ13-desaturated by FADS3 in rodents.Journal of lipid research · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConjugated linoleic acids (CLA), and principally c9t11 CLA, are suspected to have numerous preventive properties regarding non-infectious pathologies such as inflammatory diseases, atherosclerosis and several types of cancer. C9t11 CLA is produced in the rumen during biohydrogenation of linoleic acid, but can also be synthesized in mammalian tissues from trans-vaccenic acid (C18:1 t11) through the action of delta-9 desaturase (D9D). For several years, it is also known that c9t11 CLA can be synthesized from conjugated linolenic acids (CLnA), i.e. c9t11c13 CLnA and c9t11t13 CLnA. This study aimed at investigating to which extent and by which route c9t11 CLA can be produced from another isomer of CLA, the t11t13 CLA that is structurally very similar to c9t11t13 CLnA, in Caco-2 cells. METHODOLOGY/PRINCIPAL
findingsCaco-2 cells were incubated for 24 h with 20 µmol/l of t11t13 CLA in the absence or presence of sterculic oil used as an inhibitor of D9D. Caco-2 cells were able to convert t11t13 CLA into c9t11 CLA, and c9t11t13 CLnA was formed as an intermediate compound. In the presence of sterculic oil, the production of this intermediate was decreased by 46% and the formation of c9t11 CLA was decreased by 26%. No other metabolite was detected. CONCLUSIONS/SIGNIFICANCE: These results not only highlight the conversion of t11t13 CLA into c9t11 CLA but demonstrate also that this conversion involves first a desaturation step catalysed by D9D to produce c9t11t13 CLnA and then the action of another enzyme reducing the double bond on the Δ13 position.
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Registered trials
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