ArticleJournal of bacteriology2012
Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Minimizing the Anticodon-Recognized Loop ofBiomolecules · 2023Article
- Review
- Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems.Frontiers in bioengineering and biotechnology · 2020Review
- Site-Specific Incorporation of Unnatural Amino Acids into Escherichia coli Recombinant Protein: Methodology Development and Recent Achievement.Biomolecules · 2019Review
- Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications.Applied microbiology and biotechnology · 2019Review
- Translational Control using an Expanded Genetic Code.International journal of molecular sciences · 2019Review
- Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy.Journal of biomolecular NMR · 2018Article
- Chemically-defined lactose-based autoinduction medium for site-specific incorporation of non-canonical amino acids into proteins.RSC advances · 2018Article
- Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications.Journal of biological engineering · 2018Review
- Rewriting the Genetic Code.Annual review of microbiology · 2017Review
- Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins.Biotechnology and bioengineering · 2017Article
- Cell-Free Synthetic Biology: Engineering Beyond the Cell.Cold Spring Harbor perspectives in biology · 2016Review
- Emergent rules for codon choice elucidated by editing rare arginine codons in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- CRISPRi-Manipulation of Genetic Code Expansion via RF1 for Reassignment of Amber Codon in Bacteria.Scientific reports · 2016Article
- Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform.Journal of biological engineering · 2016Article
- Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli.Nucleic acids research · 2015Article
- Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon.Scientific reports · 2015Article
- Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1.Chembiochem : a European journal of chemical biology · 2015Article
- Revealing the amino acid composition of proteins within an expanded genetic code.Nucleic acids research · 2015Article
- Expanded cellular amino acid pools containing phosphoserine, phosphothreonine, and phosphotyrosine.ACS chemical biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reassigned the amber UAG stop triplet as a sense codon in Escherichia coli by expressing a UAG-decoding tRNA and knocking out the prfA gene, encoding release factor 1. UAG triplets were left at the ends of about 300 genes in the genome. In the present study, we showed that the detrimental effect of UAG reassignment could be alleviated by increasing the efficiency of UAG translation instead of reducing the number of UAGs in the genome. We isolated an amber suppressor tRNA(Gln) variant displaying enhanced suppression activity, and we introduced it into the prfA knockout strain, RFzero-q, in place of the original suppressor tRNA(Gln). The resulting strain, RFzero-q3, translated UAG to glutamine almost as efficiently as the glutamine codons, and it proliferated faster than the parent RFzero-q strain. We identified two major factors in this growth enhancement. First, the sucB gene, which is involved in energy regeneration and has two successive UAG triplets at the end, was expressed at a higher level in RFzero-q3 than RFzero-q. Second, the ribosome stalling that occurred at UAG in RFzero-q was resolved in RFzero-q3. The results revealed the importance of "backup" stop triplets, UAA or UGA downstream of UAG, to avoid the deleterious impact of UAG reassignment on the proteome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.