ArticleInvestigative ophthalmology & visual science2012
Isolation and characterization of progenitor cells in uninjured, adult rat lacrimal gland.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 59 citations in OpenAlex.
- Human Lacrimal Gland Gene Expression.PloS one · 2017Trial
- Spatial transcriptomic profiling identifies lacrimal-gland-epithelial cell-driven mechanisms underlying autoimmunity in Sjögren's disease.Frontiers in immunology · 2026Article
- Spatial transcriptomic profiling identifies lacrimal gland epithelial cell-driven mechanisms underlying autoimmunity in Sjögren's disease.bioRxiv : the preprint server for biology · 2025Article
- Epithelial and mesenchymal progenitor cells in normal and inflamed human lacrimal glands.Experimental eye research · 2025Article
- Evaluating the efficacy of stem cells in treating severe dry eye disease.World journal of stem cells · 2025Review
- Genomic DNA activates the AIM2 inflammasome and STING pathways to induce inflammation in lacrimal gland myoepithelial cells.The ocular surface · 2023Article
- ΔNp63 maintains the fidelity of the myoepithelial cell lineage and directs cell differentiation programs in the murine salivary gland.Cell death and differentiation · 2023Article
- Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies.Translational vision science & technology · 2022Article
- Article
- Lacrimal Gland Microenvironment Changes After Obstruction of Lacrimal Gland Ducts.Investigative ophthalmology & visual science · 2022Article
- Obstructive Sleep Apnea Affects Lacrimal Gland Function.Investigative ophthalmology & visual science · 2022Article
- Current approaches for the regeneration and reconstruction of ocular surface in dry eye.Frontiers in medicine · 2022Review
- Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells.Investigative ophthalmology & visual science · 2021Article
- Aquaporins 8 and 9 as Possible Markers for Adult Murine Lacrimal Gland Cells.BioMed research international · 2021Article
- Lacrimal Gland Myoepithelial Cells Are Altered in a Mouse Model of Dry Eye Disease.The American journal of pathology · 2020Article
- Origin and Lineage Plasticity of Endogenous Lacrimal Gland Epithelial Stem/Progenitor Cells.iScience · 2020Article
- Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair.Stem cell research & therapy · 2020Article
- MSC Transplantation Improves Lacrimal Gland Regeneration after Surgically Induced Dry Eye Disease in Mice.Scientific reports · 2019Article
- Three Dimensional Culture of Potential Epithelial Progenitor Cells in Human Lacrimal Gland.Translational vision science & technology · 2019Article
- Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands.Journal of visualized experiments : JoVE · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe purpose of this study was to investigate the presence of progenitor cells in the uninjured, adult rat lacrimal gland (LG).
methodsThe presence of progenitor cells was examined in LG sections from male rats using antibodies against selected stem cell markers and α-smooth muscle actin (SMA), which marks myoepithelial cells (MECs), by immunofluorescence microscopy (IF). Small, immature cells were isolated after digestion of LG with collagenase and culture in RPMI 1640 for 2 weeks. Immature cells were examined for expression of stem cell markers by IF. Immature cell were grown in neuronal, epithelial, and myoepithelial cell media, and examined by light morphology and IF using antibodies to markers of different cell lineages.
resultsIn the intact LGs, MECs expressed the stem cell markers nestin, Musashi 1, ABCG2, Pax6, Chx 10, ΔN p63, and Sox 2. All markers colocalized with SMA. Isolated immature cells contained Ki-67, nestin, Musashi 1, Pax 6, and CHX 10. In neuronal media, immature cells differentiated and assumed a neuronal cell morphology expressing neurofilament 200. In media for human corneal endothelial cells, immature cells differentiated, assumed cobblestone morphology, and labeled with the epithelial marker AE1/AE3. In RPMI media immature cells differentiated into cells with MEC-like morphology, and expressed the MEC markers SMA, α-actinin, adenylate cyclase II, and vimentin.
conclusionsWe conclude that uninjured, adult LG contains progenitor cells that may be MECs, which can be isolated and differentiated into multiple lineages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.