ArticleApplied and environmental microbiology2012
Metagenomic analysis of Streptomyces lividans reveals host-dependent functional expression.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food-Microbiota-Host Interactions in the Human Gut.International journal of molecular sciences · 2023Review
- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Current Trends in Experimental and Computational Approaches to Combat Antimicrobial Resistance.Frontiers in genetics · 2020Review
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- The art of vector engineering: towards the construction of next-generation genetic tools.Microbial biotechnology · 2019Review
- Genomic and functional techniques to mine the microbiome for novel antimicrobials and antimicrobial resistance genes.Annals of the New York Academy of Sciences · 2017Review
- Article
- Functional Genome Mining for Metabolites Encoded by Large Gene Clusters through Heterologous Expression of a Whole-Genome Bacterial Artificial Chromosome Library in Streptomyces spp.Applied and environmental microbiology · 2016Article
- Natural Product Discovery through Improved Functional Metagenomics in Streptomyces.Journal of the American Chemical Society · 2016Article
- Review
- Scarless Genome Editing and Stable Inducible Expression Vectors for Geobacter sulfurreducens.Applied and environmental microbiology · 2015Article
- Acylation of Biomolecules in Prokaryotes: a Widespread Strategy for the Control of Biological Function and Metabolic Stress.Microbiology and molecular biology reviews : MMBR · 2015Review
- Review
- Current functional metagenomic approaches only expand the existing protease sequence space, but does not presently add any novelty to it.Current microbiology · 2015Article
- Improved cultivation and metagenomics as new tools for bioprospecting in cold environments.Extremophiles : life under extreme conditions · 2015Review
- Review
- Metagenomics and novel gene discovery: promise and potential for novel therapeutics.Virulence · 2014Review
- Meta-omic characterization of prokaryotic gene clusters for natural product biosynthesis.Current opinion in biotechnology · 2013Review
- Acetoacetyl-CoA synthetase activity is controlled by a protein acetyltransferase with unique domain organization in Streptomyces lividans.Molecular microbiology · 2013Article
- Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites.Marine drugs · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most functional metagenomic studies have been limited by the poor expression of many genes derived from metagenomic DNA in Escherichia coli, which has been the predominant surrogate host to date. To expand the range of expressed genes, we developed tools for construction and functional screening of metagenomic libraries in Streptomyces lividans. We expanded on previously published protocols by constructing a system that enables retrieval and characterization of the metagenomic DNA from biologically active clones. To test the functionality of these methods, we constructed and screened two metagenomic libraries in S. lividans. One was constructed with pooled DNA from 14 bacterial isolates cultured from Alaskan soil and the second with DNA directly extracted from the same soil. Functional screening of these libraries identified numerous clones with hemolytic activity, one clone that produces melanin by a previously unknown mechanism, and one that induces the overproduction of a secondary metabolite native to S. lividans. All bioactive clones were functional in S. lividans but not in E. coli, demonstrating the advantages of screening metagenomic libraries in more than one host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.