ArticleApplied and environmental microbiology2012
Development of an engineered bioluminescent reporter phage for detection of bacterial blight of crucifers.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis.Journal of cellular and molecular medicine · 2023Article
- Three semi-selective media for Pseudomonas syringae pv. maculicola and P. cannabina pv. alisalensis.Applied microbiology and biotechnology · 2022Article
- The Bacteriophage pEp_SNUABM_08 Is a Novel Singleton Siphovirus with High Host Specificity forViruses · 2021Article
- Primers for specific detection and identification of Pseudomonas syringae pv. maculicola and P. cannabina pv. alisalensis.Applied microbiology and biotechnology · 2021Article
- The Application of Bacteriophage Diagnostics for Bacterial Pathogens in the Agricultural Supply Chain: From Farm-to-Fork.PHAGE (New Rochelle, N.Y.) · 2020Review
- Engineered Reporter Phages for Rapid Bioluminescence-Based Detection and Differentiation of ViableApplied and environmental microbiology · 2020Article
- A Syringe-Based Biosensor to Rapidly Detect Low Levels of Escherichia Coli (ECOR13) in Drinking Water Using Engineered Bacteriophages.Sensors (Basel, Switzerland) · 2020Article
- Review
- Engineering of Bacteriophages Y2::Applied and environmental microbiology · 2017Article
- Integrating recognition elements with nanomaterials for bacteria sensing.Chemical Society reviews · 2017Review
- Article
- Advances in bacteriophage-mediated control of plant pathogens.International journal of microbiology · 2012Article
- Article
Corrections and comments
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Authors and funding
6 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial blight, caused by the phytopathogen Pseudomonas cannabina pv. alisalensis, is an emerging disease afflicting important members of the Brassicaceae family. The disease is often misdiagnosed as pepper spot, a much less severe disease caused by the related pathogen Pseudomonas syringae pv. maculicola. We have developed a phage-based diagnostic that can both identify and detect the causative agent of bacterial blight and differentiate the two pathogens. A recombinant "light"-tagged reporter phage was generated by integrating bacterial luxAB genes encoding luciferase into the genome of P. cannabina pv. alisalensis phage PBSPCA1. The PBSPCA1::luxAB reporter phage is viable and stable and retains properties similar to those of the wild-type phage. PBSPCA1::luxAB rapidly and sensitively detects P. cannabina pv. alisalensis by conferring a bioluminescent signal response to cultured cells. Detection is dependent on cell viability. Other bacterial pathogens of Brassica species such as P. syringae pv. maculicola, Pseudomonas marginalis, Pectobacterium carotovorum, Xanthomonas campestris pv. campestris, and X. campestris pv. raphani either do not produce a response or produce significantly attenuated signals with the reporter phage. Importantly, the reporter phage detects P. cannabina pv. alisalensis on diseased plant specimens, indicating its potential for disease diagnosis.
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