ArticleThe Plant cell2012
The Arabidopsis ARCP protein, CSI1, which is required for microtubule stability, is necessary for root and anther development.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 59 citations in OpenAlex.
- Review
- Proteomic Analysis Reveals Accompanying Anion-Dependent Changes of Cadmium Toxicity During Arabidopsis thaliana Development.Plant, cell & environment · 2026Article
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Single-Cell Transcriptome Atlas and Dynamic Regulatory Mechanisms of Anther Development in Alfalfa (Medicago sativa L.).Plant biotechnology journal · 2025Article
- Identification of differentially expressed miRNAs between male sterile and fertile floral buds in watermelon (3 Biotech · 2024Article
- Integrated Bulk Segregant Analysis, Fine Mapping, and Transcriptome Revealed QTLs and Candidate Genes Associated with Drought Adaptation in Wild Watermelon.International journal of molecular sciences · 2023Article
- Actomyosin and CSI1/POM2 cooperate to deliver cellulose synthase from Golgi to cortical microtubules in Arabidopsis.Nature communications · 2023Article
- The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses.Plants (Basel, Switzerland) · 2021Article
- Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana.Journal of biological research (Thessalonike, Greece) · 2021Article
- The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato.Horticulture research · 2020Article
- Rice microtubule-associated protein IQ67-DOMAIN14 regulates grain shape by modulating microtubule cytoskeleton dynamics.Plant biotechnology journal · 2020Article
- The Plasma Membrane-An Integrating Compartment for Mechano-Signaling.Plants (Basel, Switzerland) · 2020Review
- The self-organization of plant microtubules inside the cell volume yields their cortical localization, stable alignment, and sensitivity to external cues.PLoS computational biology · 2018Article
- Plant Organ Shapes Are Regulated by Protein Interactions and Associations With Microtubules.Frontiers in plant science · 2018Review
- Emerging roles of cortical microtubule-membrane interactions.Journal of plant research · 2018Review
- Identification and Initial Characterization of the Effectors of an Anther Smut Fungus and Potential Host Target Proteins.International journal of molecular sciences · 2017Article
- Fine-Tuning Plant Growth in the Face of Drought.The Plant cell · 2017Article
- Protein Phosphatase 2Cs andThe Plant cell · 2017Article
- Proteomic profiling of cellulase-aid-extracted membrane proteins for functional identification of cellulose synthase complexes and their potential associated- components in cotton fibers.Scientific reports · 2016Article
- Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis.The Plant cell · 2015Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Armadillo repeat-containing proteins (ARCPs) are conserved across eukaryotic kingdoms and function in various processes. Regulation of microtubule stability by ARCPs exists widely in mammals and algae, but little is known in plants. Here, we present the functional characterization of an Arabidopsis thaliana ARCP, which was previously identified as Cellulose synthase-interactive protein1 (CSI1), and prove its crucial role in anther and root development. CSI1 is highly expressed in floral tissues, and knockout mutants of CSI1 (three allelic lines) accordingly exhibit defective anther dehiscence, which can be partially rescued by mammalian microtubule-stabilizer MAP4, suggesting that CSI1 functions by stabilizing the microtubular cytoskeleton. CSI1 binds microtubules in vitro, and immunofluorescence and coimmunoprecipitation studies confirmed the physical interactions between CSI1 and microtubules in vivo. Analysis using oryzalin, a microtubule-disrupting drug, further revealed the destabilized microtubules under CSI1 deficiency and confirmed the crucial role of CSI1 in microtubule stability. The dynamic change of CSI1 in response to dehydration strongly suggests the important function of CSI1 in dehydration-induced microtubule depolymerization and reorganization, which is crucial for anther development. These results indicate the pivotal role of CSI1 in anther development by regulating microtubule stability and hence cell morphogenesis.
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