ReviewProtein & cell2012
Emerging technology of in situ cell free expression protein microarrays.
Review in Protein & cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed.
- Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses.Scientific reports · 2017Article
- A Contra Capture Protein Array Platform for Studying Post-translationally Modified (PTM) Auto-antigenomes.Molecular & cellular proteomics : MCP · 2016Article
- Cell-free synthesis of isotopically labelled peptide ligands for the functional characterization of G protein-coupled receptors.FEBS open bio · 2016Article
- Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology.BMC research notes · 2015Article
- Nonenzymatic Protein Acetylation Detected by NAPPA Protein Arrays.ACS chemical biology · 2015Article
- Cell-free protein synthesis: the state of the art.Biotechnology letters · 2013Review
- Minireview: progress and challenges in proteomics data management, sharing, and integration.Molecular endocrinology (Baltimore, Md.) · 2012Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, in situ protein microarrays have been developed for large scale analysis and high throughput studies of proteins. In situ protein microarrays produce proteins directly on the solid surface from pre-arrayed DNA or RNA. The advances in in situ protein microarrays are exemplified by the ease of cDNA cloning and cell free protein expression. These technologies can evaluate, validate and monitor protein in a cost effective manner and address the issue of a high quality protein supply to use in the array. Here we review the importance of recently employed methods: PISA (protein in situ array), DAPA (DNA array to protein array), NAPPA (nucleic acid programmable protein array) and TUSTER microarrays and the role of these methods in proteomics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.