ArticleGenes & development2012
RpoS proteolysis is controlled directly by ATP levels in Escherichia coli.
Article in Genes & development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
- Nonlethal deleterious mutation-induced stress accelerates bacterial aging.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Proteolytic stability and aggregation in a key metabolic enzyme of bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A negative feedback loop is critical for recovery of RpoS after stress in Escherichia coli.PLoS genetics · 2024Article
- A negative feedback loop is critical for recovery of RpoS after stress inbioRxiv : the preprint server for biology · 2023Article
- Regulation of phosphate starvation-specific responses inMicrobiology (Reading, England) · 2023Review
- The activated ClpP peptidase forcefully grips a protein substrate.Biophysical journal · 2022Article
- Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli.Scientific reports · 2021Article
- Reduced ATP-dependent proteolysis of functional proteins during nutrient limitation speeds the return of microbes to a growth state.Science signaling · 2021Article
- The Non-dominant AAA+ Ring in the ClpAP Protease Functions as an Anti-stalling Motor to Accelerate Protein Unfolding and Translocation.Cell reports · 2020Article
- Transcriptomics and Proteomics Analyses of the Responses ofFrontiers in microbiology · 2020Article
- Trouble is coming: Signaling pathways that regulate general stress responses in bacteria.The Journal of biological chemistry · 2019Review
- Proteolysis mediated by the membrane-integrated ATP-dependent protease FtsH has a unique nonlinear dependence on ATP hydrolysis rates.Protein science : a publication of the Protein Society · 2019Article
- Temporal scaling of aging as an adaptive strategy ofScience advances · 2019Article
- The Role of Proteases in the Virulence of Plant Pathogenic Bacteria.International journal of molecular sciences · 2019Review
- Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli.Scientific reports · 2018Article
- Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate.Molecular cell · 2017Article
- Article
- Few regulatory metabolites coordinate expression of central metabolic genes in Escherichia coli.Molecular systems biology · 2017Article
- The physiology of growth arrest: uniting molecular and environmental microbiology.Nature reviews. Microbiology · 2016Review
- Mechanistic insights into bacterial AAA+ proteases and protein-remodelling machines.Nature reviews. Microbiology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The master regulator of stationary phase in Escherichia coli, RpoS, responds to carbon availability through changes in stability, but the individual steps in the pathway are unknown. Here we systematically block key steps of glycolysis and the citric acid cycle and monitor the effect on RpoS degradation in vivo. Nutrient upshifts trigger RpoS degradation independently of protein synthesis by activating metabolic pathways that generate small energy molecules. Using metabolic mutants and inhibitors, we show that ATP, but not GTP or NADH, is necessary for RpoS degradation. In vitro reconstitution assays directly demonstrate that ClpXP fails to degrade RpoS, but not other proteins, at low ATP hydrolysis rates. These data suggest that cellular ATP levels directly control RpoS stability.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.