ArticleRestorative neurology and neuroscience2012
Effect of aging on 5-hydroxymethylcytosine in the mouse hippocampus.
Article in Restorative neurology and neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 130 citations in OpenAlex.
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- DNA Methylation: A Promising Approach in Management of Alzheimer's Disease and Other Neurodegenerative Disorders.Biology · 2022Review
- Brain Region- and Age-Dependent 5-Hydroxymethylcytosine Activity in the Non-Human Primate.Frontiers in aging neuroscience · 2022Article
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- Cellular hallmarks of aging emerge in the ovary prior to primordial follicle depletion.Mechanisms of ageing and development · 2021Article
- DNA methylation methods: Global DNA methylation and methylomic analyses.Methods (San Diego, Calif.) · 2021Review
- Alterations in the Genomic Distribution of 5hmC in In Vivo Aged Human Skin Fibroblasts.International journal of molecular sciences · 2020Article
- DeepH&M: Estimating single-CpG hydroxymethylation and methylation levels from enrichment and restriction enzyme sequencing methods.Science advances · 2020Article
- Tet1 Deficiency Leads to Premature Reproductive Aging by Reducing Spermatogonia Stem Cells and Germ Cell Differentiation.iScience · 2020Article
- Epigenetic mechanisms of neurodegenerative diseases and acute brain injury.Neurochemistry international · 2020Review
- Global modulation in DNA epigenetics during pro-inflammatory macrophage activation.Epigenetics · 2019Article
- The strand-biased mitochondrial DNA methylome and its regulation by DNMT3A.Genome research · 2019Article
- Decreased global DNA hydroxymethylation in neural tube defects: Association with polycyclic aromatic hydrocarbons.Epigenetics · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeAging is believed to affect epigenetic marking of brain DNA with 5-methylcytosine (5mC) and possibly via the 5mC to 5-hydroxymethylcytosine (5hmC) conversion by TET (ten-eleven translocation) enzymes. We investigated the impact of aging on hippocampal DNA 5-hydroxymethylation including in the sequence of aging-susceptible 5-lipoxygenase (5-LOX) gene.
methodsHippocampal samples were obtained from C57BL6 mice. Cellular 5hmC localization was determined by immunofluorescence. The global 5mC and 5hmC contents were measured with the corresponding ELISA. The 5-LOX 5hmC content was measured using a glucosyltransferase/enzymatic restriction digest assay. TET mRNA was measured using qRT-PCR.
resultsGlobal hippocampal 5hmC content increased during aging as did the 5hmC content in the 5-LOX gene. This occurred without alterations of TET1-3 mRNAs and without changes in the content of 8-hydroxy-2-deoxy-guanosine, a marker of non-enzymatic DNA oxidation.
conclusionsThe aging-associated increase of hippocampal 5hmC content (global and 5-LOX) appears to be unrelated to oxidative stress. It may be driven by an altered activity but not by the increased expression of the three TET enzymes. Global 5hmC content was increased during aging in the absence of 5mC decrease, suggesting that 5hmC could act as an epigenetic marker and not only as an intermediary in DNA demethylation. Further research is needed to elucidate the functional implications of the impact of aging on hippocampal cytosine hydroxymethylation.
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