ArticleDevelopmental biology2012
The sperm surface localization of the TRP-3/SPE-41 Ca2+ -permeable channel depends on SPE-38 function in Caenorhabditis elegans.
Article in Developmental biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- The Caenorhabditis elegans spe-21 gene that encodes a palmitoyltransferase is necessary for spermiogenesis.Developmental biology · 2025Article
- CRISPR/Cas9 Mediated Fluorescent Tagging ofBiomolecules · 2023Article
- Eukaryotic fertilization and gamete fusion at a glance.Journal of cell science · 2022Article
- The molecular underpinnings of fertility: Genetic approaches inAdvanced genetics (Hoboken, N.J.) · 2021Article
- Article
- Developmental Control of the Cell Cycle: Insights fromGenetics · 2019Review
- Decoding the intensity of sensory input by two glutamate receptors in one C. elegans interneuron.Nature communications · 2018Article
- Caenorhabditis elegans sperm membrane protein interactome.Biology of reproduction · 2018Article
- Article
- The Immunoglobulin-like Gene spe-45 Acts during Fertilization in Caenorhabditis elegans like the Mouse Izumo1 Gene.Current biology : CB · 2015Article
- SLC6 family transporter SNF-10 is required for protease-mediated activation of sperm motility in C. elegans.Developmental biology · 2014Article
- The regulation of spermatogenesis and sperm function in nematodes.Seminars in cell & developmental biology · 2014Review
- Calcium signaling surrounding fertilization in the nematode Caenorhabditis elegans.Cell calcium · 2013Review
- Thermosensitive ion channel TRPV1 is endogenously expressed in the sperm of a fresh water teleost fish (Labeo rohita) and regulates sperm motility.Channels (Austin, Tex.)Article
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7 authors at 2 institutions in 1 country.
Funding
Abstract
Despite undergoing normal development and acquiring normal morphology and motility, mutations in spe-38 or trp-3/spe-41 cause identical phenotypes in Caenorhabditis elegans-mutant sperm fail to fertilize oocytes despite direct contact. SPE-38 is a novel, four-pass transmembrane protein and TRP-3/SPE-41 is a Ca(2+)-permeable channel. Localization of both of these proteins is confined to the membranous organelles (MOs) in undifferentiated spermatids. In mature spermatozoa, SPE-38 is localized to the pseudopod and TRP-3/SPE-41 is localized to the whole plasma membrane. Here we show that the dynamic redistribution of TRP-3/SPE-41 from MOs to the plasma membrane is dependent on SPE-38. In spe-38 mutant spermatozoa, TRP-3/SPE-41 is trapped within the MOs and fails to reach the cell surface despite MO fusion with the plasma membrane. Split-ubiquitin yeast-two-hybrid analyses revealed that the cell surface localization of TRP-3/SPE-41 is likely regulated by SPE-38 through a direct protein-protein interaction mechanism. We have identified sequences that influence the physical interaction between SPE-38 and TRP-3/SPE-41, and show that these sequences in SPE-38 are required for fertility in transgenic animals. Despite the mislocalization of TRP-3/SPE-41 in spe-38 mutant spermatozoa, ionomycin or thapsigargin induced influx of Ca(2+) remains unperturbed. This work reveals a new paradigm for the regulated surface localization of a Ca(2+)-permeable channel.
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