ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2012
Rapid and reliable quantitation of amino acids and myo-inositol in mouse brain by high performance liquid chromatography and tandem mass spectrometry.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- Accumulated BCAAs and BCKAs contribute to the HFD-induced deterioration of Alzheimer's disease via a dysfunctional TREM2-related reduction in microglial β-amyloid clearance.Journal of neuroinflammation · 2024Article
- Development and validation of an LC‒MS/MS method for the determination of cyclocreatine phosphate and its related endogenous biomolecules in rat heart tissues.BMC chemistry · 2024Article
- Overview of Inositol and Inositol Phosphates on Chemoprevention of Colitis-Induced Carcinogenesis.Molecules (Basel, Switzerland) · 2020Review
- Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation ofAnimals : an open access journal from MDPI · 2020Article
- Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry.Plant methods · 2019Article
- Quantification of eicosanoids and their metabolites in biological matrices: a review.Bioanalysis · 2018Review
- d-Amino Acid Levels in Perfused Mouse Brain Tissue and Blood: A Comparative Study.ACS chemical neuroscience · 2017Article
- Brain region mapping using global metabolomics.Chemistry & biology · 2014Article
- Antenatal taurine supplementation increases taurine content in intrauterine growth restricted fetal rat brain tissue.Metabolic brain disease · 2014Article
- Combinatorial assessments of brain tissue metabolomics and histopathology in rodent models of human immunodeficiency virus infection.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15 min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20 ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000 ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.