ArticleVeterinary immunology and immunopathology2012
Generation and characterization of monoclonal antibodies to equine CD16.
Article in Veterinary immunology and immunopathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Immunohistological characterization of equine synovial tissue in metacarpophalangeal joints of different ages and osteoarthritis status.Osteoarthritis and cartilage open · 2026Article
- Equine models in translational medicine: A comparative approach to human health.Animal models and experimental medicine · 2026Review
- IgE-binding monocytes upregulate the coagulation cascade in allergic horses.Genes and immunity · 2023Article
- Advantages and Challenges of Differential Immune Cell Count Determination in Blood and Milk for Monitoring the Health and Well-Being of Dairy Cows.Veterinary sciences · 2022Review
- Comprehensive Flow Cytometric Characterization of Bronchoalveolar Lavage Cells Indicates Comparable Phenotypes Between Asthmatic and Healthy Horses But Functional Lymphocyte Differences.Frontiers in immunology · 2022Article
- IgE-Binding Monocytes Have an Enhanced Ability to Produce IL-8 (CXCL8) in Animals with Naturally Occurring Allergy.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Single-cell resolution landscape of equine peripheral blood mononuclear cells reveals diverse cell types including T-betBMC biology · 2021Article
- Phenotype and function of IgE-binding monocytes in equine Culicoides hypersensitivity.PloS one · 2020Article
- Article
- Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.Veterinary research · 2015Article
- The equine alveolar macrophage: functional and phenotypic comparisons with peritoneal macrophages.Veterinary immunology and immunopathology · 2013Article
- Generation and characterization of monoclonal antibodies to equine NKp46.Veterinary immunology and immunopathology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The low-affinity Fc receptor CD16 plays a central role in the inflammatory and innate immune responses of many species, but has not yet been investigated in the horse. Using the predicted extracellular region of equine CD16 expressed as a recombinant fusion protein with equine IL-4 (rIL-4/CD16), we generated a panel of mouse monoclonal antibodies (mAbs) that recognize equine CD16. Nine mAbs were chosen for characterization based upon recognition of CD16, but not IL-4, in ELISA. All nine mAbs recognized full-length, cell-surface CD16 expressed as a GFP fusion protein by CHO cells, but not the closely related Fc receptor CD32 expressed in the same system. In flow cytometric analysis with equine peripheral leukocytes, the mAbs labeled cells in the granulocyte, monocyte, and lymphocyte populations in a pattern consistent with other species. Monocytes that were strongly labeled with CD16 mAb 9G5 were also positive for the LPS receptor CD14. Cytospins made with peripheral leukocytes were immunohistochemically labeled and showed mAb recognition of primarily mononuclear cells. ELISA revealed that the nine mAbs can be grouped into three patterns of epitope recognition. These new antibodies will serve as useful tools in the investigation of equine immune responses and inflammatory processes.
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Registered trials
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