ArticleChemistry Central journal2012
Glucose metabolite glyoxal induces senescence in telomerase-immortalized human mesenchymal stem cells.
Article in Chemistry Central journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Effects of advanced glycation end products (AGEs) on the differentiation potential of primary stem cells: a systematic review.Stem cell research & therapy · 2023Pooled it
- Establishment of Three Different Glycation-Damage Cell Models and Analysis of Their Action Mechanism.Metabolites · 2026Article
- The Peroxidation of Lipids, Cellular Senescence and Aging.Journal of aging science · 2024Article
- Advanced glycation end product (AGE) targeting antibody SIWA318H is efficacious in preclinical models for pancreatic cancer.Scientific reports · 2023Article
- Hyperbaric oxygen therapy efficacy on mandibular defect regeneration in rats with diabetes mellitus: an animal study.BMC oral health · 2023Article
- Non-Obese MKR Mouse Model of Type 2 Diabetes Reveals Skeletal Alterations in Mineralization and Material Properties.JBMR plus · 2022Article
- An essay on the nominal vs. real definitions of aging.Biogerontology · 2021Article
- DNA damage response manages cell cycle restriction of senile multipotent mesenchymal stromal cells.Molecular biology reports · 2020Review
- Targeting Cell Senescence for the Treatment of Age-Related Bone Loss.Current osteoporosis reports · 2019Review
- Obesity and type-2 diabetes as inducers of premature cellular senescence and ageing.Biogerontology · 2018Review
- Investigating the Glycating Effects of Glucose, Glyoxal and Methylglyoxal on Human Sperm.Scientific reports · 2018Article
- Hypoxia induces senescence of bone marrow mesenchymal stem cells via altered gut microbiota.Nature communications · 2018Article
- Cytocompatible Catalyst-Free Photodegradable Hydrogels for Light-Mediated RNA Release To Induce hMSC Osteogenesis.ACS biomaterials science & engineering · 2017Article
- Light-triggered RNA release and induction of hMSC osteogenesis via photodegradable, dual-crosslinked hydrogels.Nanomedicine (London, England) · 2016Article
- Tetraploidization or autophagy: The ultimate fate of senescent human endometrial stem cells under ATM or p53 inhibition.Cell cycle (Georgetown, Tex.) · 2016Article
- Cellular senescence: from growth arrest to immunogenic conversion.Age (Dordrecht, Netherlands) · 2015Review
- Tissue resident stem cells: till death do us part.Biogerontology · 2013Review
- Biphasic Dose-Response and Hormetic Effects of Stress Hormone Hydrocortisone on Telomerase-Immortalized Human Bone Marrow Stem Cells In Vitro.Dose-response : a publication of International Hormesis SocietyArticle
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVarious by-products of the cellular metabolism, such as reactive carbonyl species (RCS) are potentially harmful to cells and tissues, and play a role in many physiological and pathological processes. Among various RCS is the highly reactive dicarbonyl glyoxal (GO), which is a natural physiological metabolite produced by the auto-oxidation of glucose, and can form covalent adducts known as advanced glycation endproducts (AGE). We have previously reported that GO accelerates ageing and causes premature senescence in normal human skin fibroblasts.
resultsUsing a bone marrow-derived telomerase-immortalised mesenchymal stem cell line hMSC-TERT we have observed that an exposure of cells to 0.75 mM and 1 mM GO induces irreversible cellular senescence within 3 days. Induction of senescence in hMSC-TERT was demonstrated by a variety of markers, including characteristic cell morphology and enlargement, vacuolisation, multinucleation, induction of senescence associated β-galactosidase, cell cycle arrest, and increased levels of a cell cycle inhibitor p16. These changes were accompanied by increased extent of DNA breaks as measured by the comet assay, and increased levels of the AGE product, carboxymethyl-lysine (CML). Furthermore, the in vitro differentiation potential of hMSC-TERT to become functional osteoblasts was highly reduced in GO-treated stem cells, as determined by alkaline phosphatase (ALP) activity and mineralized matrix (MM) formation.
conclusionsThe results of our study imply that an imbalanced glucose metabolism can reduce the functioning ability of stem cells in vivo both during ageing and during stem cell-based therapeutic interventions.
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Registered trials
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