ArticleJournal of clinical microbiology2012
Standardized one-step real-time reverse transcription-PCR assay for universal detection and quantification of hepatitis delta virus from clinical samples in the presence of a heterologous internal-control RNA.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Laboratory development of an RNA quantitative RT-PCR assay reporting in international units for hepatitis D virus.Frontiers in microbiology · 2024Article
- Reflex testing automatization for HBsAg carriers: A novel era for hepatitis delta management.JHEP reports : innovation in hepatology · 2024Article
- CRISPR/Cas13a-Assisted accurate and portable hepatitis D virus RNA detection.Emerging microbes & infections · 2023Article
- Diagnosis of HDV: From virology to non-invasive markers of fibrosis.Liver international : official journal of the International Association for the Study of the Liver · 2023Review
- Identification of selective hepatitis delta virus ribozyme inhibitors by high-throughput screening of small molecule libraries.JHEP reports : innovation in hepatology · 2023Article
- Viral Diagnosis of Hepatitis B and Delta: What We Know and What Is Still Required? Specific Focus on Low- and Middle-Income Countries.Microorganisms · 2022Review
- Hepatitis D: challenges in the estimation of true prevalence and laboratory diagnosis.Gut pathogens · 2021Review
- Subclinical and Clinical Outcomes in Patients Coinfected With HIV and Chronic Hepatitis B Virus From Clinical Outpatient Centers in France: Protocol for an Ambispective, Longitudinal Cohort Study.JMIR research protocols · 2021Article
- Use of chimeric influenza viruses as a novel internal control for diagnostic rRT-PCR assays.Applied microbiology and biotechnology · 2016Article
- Hepatitis D Virus: A Call to Screening.Gastroenterology & hepatology · 2014Article
- Relevance of a full-length genomic RNA standard and a thermal-shock step for optimal hepatitis delta virus quantification.Journal of clinical microbiology · 2014Article
- Serological and molecular diagnosis of hepatitis delta virus infection: results of a French national quality control study.Journal of clinical microbiology · 2014Article
- Effect of tenofovir with and without interferon on hepatitis D virus replication in HIV-hepatitis B virus-hepatitis D virus-infected patients.AIDS research and human retroviruses · 2013Article
- Hepatitis delta virus: a peculiar virus.Advances in virology · 2013Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As for other chronic viral diseases, quantification of hepatitis delta virus (HDV) loads may be useful for patient management. We describe a one-step quantitative reverse transcription-PCR assay that is reliable and automatable and meets the regulatory authorities' standards for accurate quantification of the major HDV genotypes. It includes an internal control and uses in vitro-transcribed RNAs as standards. Its linearity range is 500 to 1.7 × 10(11) copies/ml, its sensitivity is around 150 copies/ml, its repeatability is around 15%, and its reproducibility is below 0.25 log(10) copies/ml.
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