ArticleJournal of clinical microbiology2012
Development and evaluation of a genus-specific, probe-based, internal-process-controlled real-time PCR assay for sensitive and specific detection of Blastocystis spp.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Epidemiology of Blastocystis sp. infection in China: a systematic review.Parasite (Paris, France) · 2019Pooled it
- Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial.Parasites & vectors · 2023Trial
- MappingOne health (Amsterdam, Netherlands) · 2026Article
- Assessment of the Therapeutic Role of Allium tuncelianum Extract in Rats Infected with Blastocystis Subtype 3.Acta parasitologica · 2025Article
- Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single-Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as 'Non-Pathogenic'.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Review
- Diet Quality Modulates Gut Microbiota Structure inMicroorganisms · 2025Article
- Epidemiological Exploration ofVeterinary sciences · 2025Article
- Impact of Blastocystis carriage and colonization intensity on gut microbiota composition in a non-westernized rural population from Colombia.PLoS neglected tropical diseases · 2025Article
- Implementation of real-time PCR assays for diagnosing intestinal protozoa infections.Parasitology research · 2025Article
- A cross - sectional investigation on the molecular infection rate and subtype distribution ofFrontiers in cellular and infection microbiology · 2025Article
- Gastrointestinal Parasites in Non-Human Primates in Zoological Gardens in Northern Italy.Animals : an open access journal from MDPI · 2024Article
- Clustering of Gastrointestinal Microorganisms in Human Stool Samples from Ghana.Pathogens (Basel, Switzerland) · 2024Article
- Subtype Distribution ofMicroorganisms · 2024Article
- Prevalence of Blastocystis and Dientamoeba fragilis in diarrheal patients in Corum, Türkiye.Parasitology research · 2023Article
- Protozoan parasites and free-living amoebae contamination in organic leafy green vegetables and strawberries from Spain.Food and waterborne parasitology · 2023Article
- Differential gut microbiome in spondyloarthritis patients associated to Blastocystis colonization.Scientific reports · 2023Article
- Investigation of intestinal parasites by conventional and molecular methods in children with gastrointestinal system complaints.Parasitology research · 2023Article
- First identification of Blastocystis sp. subtypes in Rex rabbits in China.Parasitology research · 2023Article
- Decreased fecal calprotectin levels in Spondyloarthritis patients colonized by Blastocystis spp.Scientific reports · 2022Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blastocystis is a common intestinal parasite of unsettled clinical significance, which is not easily detected by standard parasitological methods. The genus comprises at least 13 subtypes (STs) (which likely represent separate species), 9 of which have been found in humans. Recent data indicate that at least one of the subtypes is associated with intestinal disease. A quantitative TaqMan 5' nuclease real-time PCR (TaqMan PCR) including an internal process control (IPC) was developed for the detection of Blastocystis and shown to be applicable to genomic DNAs extracted directly from feces. The assay enabled successful amplification of DNAs from all relevant subtypes within the genus (ST1 to ST9). For assay evaluation, 153 samples previously tested by xenic in vitro culture (XIVC) were screened by the TaqMan assay. A total of 49/51 samples positive by XIVC and 13/102 samples negative by XIVC were positive by the TaqMan assay; samples positive by the TaqMan assay and negative by XIVC were subsequently tested by conventional PCR, and amplicons could be identified to the subtype level by sequencing in 69% of the cases. Compared to the TaqMan assay, XIVC had a sensitivity of 79%. This is the first time that a genus-specific, probe-based, internal-process-controlled real-time PCR assay for the detection Blastocystis has been introduced.
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