ArticleNucleic acids research2012
High-throughput single-molecule analysis of DNA-protein interactions by tethered particle motion.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- Single-molecule calorimeter and free energy landscape.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Critical Review: digital resolution biomolecular sensing for diagnostics and life science research.Lab on a chip · 2020Review
- Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP.Nature communications · 2020Article
- Sequence-dependent dynamics of synthetic and endogenous RSSs in V(D)J recombination.Nucleic acids research · 2020Article
- Roles of entropic and solvent damping forces in the dynamics of polymer tethered nanoparticles and implications for single molecule sensing.Chemical science · 2020Article
- Model-free 3D localization with precision estimates for brightfield-imaged particles.Optics express · 2019Article
- Tethered multifluorophore motion reveals equilibrium transition kinetics of single DNA double helices.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Continuous biomarker monitoring by particle mobility sensing with single molecule resolution.Nature communications · 2018Article
- Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination.Micromachines · 2018Review
- How does temperature impact the conformation of single DNA molecules below melting temperature?Nucleic acids research · 2018Article
- A mechanistic study of helicases with magnetic traps.Protein science : a publication of the Protein Society · 2017Review
- Refining particle positions using circular symmetry.PloS one · 2017Article
- Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics.Methods in enzymology · 2017Article
- FtsK translocation permits discrimination between an endogenous and an imported Xer/dif recombination complex.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Label-free DNA sequencing using Millikan detection.Analytical biochemistry · 2015Article
- Probing a label-free local bend in DNA by single molecule tethered particle motion.Nucleic acids research · 2015Article
- Direct observation of the translocation mechanism of transcription termination factor Rho.Nucleic acids research · 2015Article
- High-throughput single-molecule studies of protein-DNA interactions.FEBS letters · 2014Review
- TPM analyses reveal that FtsK contributes both to the assembly and the activation of the XerCD-dif recombination synapse.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tethered particle motion (TPM) monitors the variations in the effective length of a single DNA molecule by tracking the Brownian motion of a bead tethered to a support by the DNA molecule. Providing information about DNA conformations in real time, this technique enables a refined characterization of DNA-protein interactions. To increase the output of this powerful but time-consuming single-molecule assay, we have developed a biochip for the simultaneous acquisition of data from more than 500 single DNA molecules. The controlled positioning of individual DNA molecules is achieved by self-assembly on nanoscale arrays fabricated through a standard microcontact printing method. We demonstrate the capacity of our biochip to study biological processes by applying our method to explore the enzymatic activity of the T7 bacteriophage exonuclease. Our single molecule observations shed new light on its behaviour that had only been examined in bulk assays previously and, more specifically, on its processivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.