ArticleBlood2012
Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.
Article in Blood, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Retinoblastoma gene expression profiling based on bioinformatics analysis.BMC medical genomics · 2023Article
- Efficient terminal erythroid differentiation requires the APC/C cofactor Cdh1 to limit replicative stress in erythroblasts.Scientific reports · 2022Article
- Tipping the balance between erythroid cell differentiation and induction of anemia in response to the inflammatory pathology associated with chronic trypanosome infections.Frontiers in immunology · 2022Article
- Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis.Scientific reports · 2021Article
- Identification of Candidate Signature Genes and Key Regulators Associated With Trypanotolerance in the Sheko Breed.Frontiers in genetics · 2019Article
- Silencing of E2F3 suppresses tumor growth of Her2+ breast cancer cells by restricting mitosis.Oncotarget · 2015Article
- Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality.Nature communications · 2015Article
- Repression by RB1 characterizes genes involved in the penultimate stage of erythroid development.Cell cycle (Georgetown, Tex.) · 2015Article
- Involvement of atypical transcription factor E2F8 in the polyploidization during mouse and human decidualization.Cell cycle (Georgetown, Tex.) · 2015Article
- Transcriptional control of stem cell fate by E2Fs and pocket proteins.Frontiers in genetics · 2015Review
- Inactivation of Rb and E2f8 synergizes to trigger stressed DNA replication during erythroid terminal differentiation.Molecular and cellular biology · 2014Article
- E2F activators signal and maintain centrosome amplification in breast cancer cells.Molecular and cellular biology · 2014Article
- Mx1-cre mediated Rgs12 conditional knockout mice exhibit increased bone mass phenotype.Genesis (New York, N.Y. : 2000) · 2013Article
- Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling.Blood cancer journal · 2013Article
- Characterization of ENU-induced Mutations in Red Blood Cell Structural Proteins.Computational and structural biotechnology journal · 2013Article
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Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The retinoblastoma (Rb) tumor suppressor plays important roles in regulating hematopoiesis, particularly erythropoiesis. In an effort to understand whether Rb function can be mediated by E2F transcription factors in a BM-derived hematopoietic system in mice, we uncovered a functional synergy between Rb and E2F8 to promote erythropoiesis and to prevent anemia. Specifically, whereas Mx1-Cre-mediated inactivation of Rb or E2f8 in hematopoietic stem cells only led to mild erythropoietic defects, concomitant inactivation of both genes resulted in marked ineffective erythropoiesis and mild hemolysis, leading to severe anemia despite the presence of enhanced extramedullary erythropoiesis. Interestingly, although ineffective erythropoiesis was already present in the RbΔ/Δ mice and exacerbated in the RbΔ/Δ;E2f8Δ/Δ mice, hemolysis was exclusively manifested in the double-knockout mice. Using an adoptive transfer system and an erythroid-specific knockout system, we have shown that the synergy of Rb and E2f8 deficiency in triggering severe anemia is intrinsic to the erythroid lineage. Surprisingly, concomitant inactivation of Rb and E2f7, a close family member of E2f8, did not substantially worsen the erythropoietic defect resulted from Rb deficiency. The results of the present study reveal the specificity of E2F8 in mediating Rb function in erythropoiesis and suggest critical and overlapping roles of Rb and E2f8 in maintaining normal erythropoiesis and in preventing hemolysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.