ArticleInternational journal of epidemiology2012
DNA methylation shows genome-wide association of NFIX, RAPGEF2 and MSRB3 with gestational age at birth.
Article in International journal of epidemiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 6 of them syntheses that pooled it.
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The trial behind it
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Who cites it
47 citing papers in PubMed, 6 syntheses or guidelines pooled it, 75 citations in OpenAlex.
- Sex-based differences in placental DNA methylation profiles related to gestational age: an NIH ECHO meta-analysis.Epigenetics · 2023Pooled it
- Racial and ethnic representation in epigenomic studies of preterm birth: a systematic review.Epigenomics · 2021Pooled it
- An epigenome-wide association study of early-onset major depression in monozygotic twins.Translational psychiatry · 2020Pooled it
- Epigenome-wide meta-analysis of blood DNA methylation in newborns and children identifies numerous loci related to gestational age.Genome medicine · 2020Pooled it
- Associations between body size, nutrition and socioeconomic position in early life and the epigenome: A systematic review.PloS one · 2018Pooled it
- A Systematic Review of DNA Methylation and Preterm Birth in African American Women.Biological research for nursing · 2017Pooled it
- Identification of epigenetic memory candidates associated with gestational age at birth through analysis of methylome and transcriptional data.Scientific reports · 2021Trial
- Exploring Differentially Methylated Genes among Preterm Birth and Full-Term Birth.Lifestyle genomics · 2025Article
- Caucasian and African American racial disparity in neonatal hospital mortality.Frontiers in pediatrics · 2024Article
- The Role of Genetics in Preterm Birth.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Emerging role of toll-like receptors signaling and its regulators in preterm birth: a narrative review.Archives of gynecology and obstetrics · 2023Review
- Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor.Communications biology · 2023Article
- Nucleated red blood cells explain most of the association between DNA methylation and gestational age.Communications biology · 2023Article
- Adulthood blood levels of hsa-miR-29b-3p associate with preterm birth and adult metabolic and cognitive health.Scientific reports · 2021Article
- Gaining a deeper understanding of social determinants of preterm birth by integrating multi-omics data.Pediatric research · 2021Review
- Replicated umbilical cord blood DNA methylation loci associated with gestational age at birth.Epigenetics · 2020Article
- Epigenetic biomarkers and preterm birth.Environmental epigenetics · 2020Review
- Analysis of two birth tissues provides new insights into the epigenetic landscape of neonates born preterm.Clinical epigenetics · 2019Observational
- Exploring insertions and deletions (indels) ofArchives animal breeding · 2019Article
- Genome-wide association analysis uncovers variants for reproductive variation across dog breeds and links to domestication.Evolution, medicine, and public health · 2019Article
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
Abstract
backgroundGestational age at birth strongly predicts neonatal, adolescent and adult morbidity and mortality through mostly unknown mechanisms. Identification of specific genes that are undergoing regulatory change prior to birth, such as through changes in DNA methylation, would increase our understanding of developmental changes occurring during the third trimester and consequences of pre-term birth (PTB).
methodsWe performed a genome-wide analysis of DNA methylation (using microarrays, specifically CHARM 2.0) in 141 newborns collected in Baltimore, MD, using novel statistical methodology to identify genomic regions associated with gestational age at birth. Bisulphite pyrosequencing was used to validate significant differentially methylated regions (DMRs), and real-time PCR was performed to assess functional significance of differential methylation in a subset of newborns.
resultsWe identified three DMRs at genome-wide significance levels adjacent to the NFIX, RAPGEF2 and MSRB3 genes. All three regions were validated by pyrosequencing, and RAGPEF2 also showed an inverse correlation between DNA methylation levels and gene expression levels. Although the three DMRs appear very dynamic with gestational age in our newborn sample, adult DNA methylation levels at these regions are stable and of equal or greater magnitude than the oldest neonate, directionally consistent with the gestational age results.
conclusionsWe have identified three differentially methylated regions associated with gestational age at birth. All three nearby genes play important roles in the development of several organs, including skeletal muscle, brain and haematopoietic system. Therefore, they may provide initial insight into the basis of PTB's negative health outcomes. The genome-wide custom DNA methylation array technology and novel statistical methods employed in this study could constitute a model for epidemiologic studies of epigenetic variation.
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