ArticleNature protocols2012
Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populations.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Micro Pattern-Based 3D Cell Culture Platform: An Overview of Technologies and Applications.Exploration (Beijing, China) · 2026Review
- Effect of 2D and 3D ECM and Biomechanical Cues on Human iPSC-Derived Liver Progenitor Cell Differentiation.Advanced healthcare materials · 2026Article
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Effect of distinct ECM microenvironments on the genome-wide chromatin accessibility and gene expression responses of hepatic stellate cells.Acta biomaterialia · 2023Article
- Delineating cooperative effects of Notch and biomechanical signals on patterned liver differentiation.Communications biology · 2022Article
- Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.ACS biomaterials science & engineering · 2022Article
- Droplet Microarray Based Screening Identifies Proteins for Maintaining Pluripotency of hiPSCs.Advanced healthcare materials · 2022Article
- Application and prospects of high-throughput screening forWorld journal of stem cells · 2022Review
- Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.Advanced healthcare materials · 2022Article
- Spatial Regulation of Valve Interstitial Cell Phenotypes within Three-Dimensional Micropatterned Hydrogels.ACS biomaterials science & engineering · 2019Article
- Article
- Substrate stiffness and VE-cadherin mechano-transduction coordinate to regulate endothelial monolayer integrity.Biomaterials · 2017Article
- Combinatorial Extracellular Matrix Microenvironments for Probing Endothelial Differentiation of Human Pluripotent Stem Cells.Scientific reports · 2017Article
- A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces.Journal of visualized experiments : JoVE · 2017Article
- Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia.Acta biomaterialia · 2016Article
- Polymer microarray technology for stem cell engineering.Acta biomaterialia · 2016Review
- Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFβ, and extracellular matrix.Scientific reports · 2016Article
- Article
- In vivo high-content evaluation of three-dimensional scaffolds biocompatibility.Tissue engineering. Part C, Methods · 2014Article
- Capillary Network-Like Organization of Endothelial Cells in PEGDA Scaffolds Encoded with Angiogenic SignalsAdvanced functional materials · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the development of an organism, cells are exposed to a myriad of signals, structural components and scaffolds, which collectively make up the cellular microenvironment. The majority of current developmental biology studies examine the effect of individual or small subsets of molecules and parameters on cellular behavior, and they consequently fail to explore the complexity of factors to which cells are exposed. Here we describe a technology, referred to as arrayed cellular microenvironments (ACMEs), that allows for a high-throughput examination of the effects of multiple extracellular components in a combinatorial manner on any cell type of interest. We will specifically focus on the application of this technology to human pluripotent stem cells (hPSCs), a population of cells with tremendous therapeutic potential, and one for which growth and differentiation conditions are poorly characterized and far from defined and optimized. A standard ACME screen uses the technologies previously applied to the manufacture and analysis of DNA microarrays, requires standard cell-culture facilities and can be performed from beginning to end within 5-10 days.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.