ArticleMolecular biotechnology2013
Nitrogen starvation, salt and heat stress in coffee (Coffea arabica L.): identification and validation of new genes for qPCR normalization.
Article in Molecular biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Reference gene selection for gene expression analysis in Coffea arabica L. under ABA and gibberellin treatments.Molecular biology reports · 2026Article
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- Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns ofInternational journal of molecular sciences · 2023Article
- Constitutive Defense Strategy of Coffee Under Field Conditions: A Comparative Assessment of Resistant and Susceptible Cultivars to Rust.Molecular biotechnology · 2022Article
- Transcriptomic alterations in roots of two contrastingFrontiers in genetics · 2022Article
- Crosstalk Between Ethylene and Abscisic Acid During Changes in Soil Water Content Reveals a New Role for 1-Aminocyclopropane-1- Carboxylate in Coffee Anthesis Regulation.Frontiers in plant science · 2022Article
- LysM receptors in Coffea arabica: Identification, characterization, and gene expression in response to Hemileia vastatrix.PloS one · 2022Article
- The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Low-Copy Genes in Terpenoid Metabolism: The Evolution and Expression ofPlants (Basel, Switzerland) · 2020Article
- Identification and evaluation of reference genes for quantitative real-time PCR analysis in Passiflora edulis under stem rot condition.Molecular biology reports · 2020Article
- An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses.Functional & integrative genomics · 2019Article
- Primer design and amplification efficiencies are crucial for reliability of quantitative PCR studies of caffeine biosynthetic3 Biotech · 2018Article
- Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR.Plant methods · 2018Article
- Identification of Endogenous Reference Genes for RT-qPCR Expression Analysis in Urochloa brizantha Under Abiotic Stresses.Scientific reports · 2017Article
- Selection and Validation of Reference Genes for Accurate RT-qPCR Data Normalization inFrontiers in plant science · 2017Article
- Validation of Suitable Reference Genes for RT-qPCR Data inFrontiers in plant science · 2017Article
- Genome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffea.Genetics and molecular biology · 2017Article
- Article
- Transcriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes in leaves and fruits.Molecular genetics and genomics : MGG · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Abiotic stresses are among the most important factors that affect food production. One important step to face these environmental challenges is the transcriptional modulation. Quantitative real-time PCR is a rapid, sensitive, and reliable method for the detection of mRNAs and it has become a powerful tool to mitigate plant stress tolerance; however, suitable reference genes are required for data normalization. Reference genes for coffee plants during nitrogen starvation, salinity and heat stress have not yet been reported. We evaluated the expression stability of ten candidate reference genes using geNorm PLUS, NormFinder, and BestKeeper softwares, in plants submitted to nitrogen starvation, salt and heat stress. EF1, EF1α, GAPDH, MDH, and UBQ10 were ranked as the most stable genes in all stresses and software analyses, while RPL39 and RPII were classified as the less reliable references. For reference gene validation, the transcriptional pattern of a Coffea non-symbiotic hemoglobin (CaHb1) was analyzed using the two new recommended and the most unstable gene references for normalization. The most unstable gene may lead to incorrect interpretation of CaHb1 transcriptional analysis. Here, we recommend two new reference genes in Coffea for use in data normalization in abiotic stresses: MDH and EF1.
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