ArticleProceedings of the National Academy of Sciences of the United States of America2012
Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers, 1 of them a synthesis that pooled it.
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Who cites it
111 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Calorie restriction does not elicit a robust extension of replicative lifespan in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2014Pooled it
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- BudFinder: A Masked Auto-Encoder vision transformer framework for yeast budding detection and lifespan quantification.PLoS computational biology · 2026Article
- Single-cell aging trajectories reveal a dynamic coupling between nuclear size and proteasome concentration.iScience · 2026Article
- Cdc42 interacts with chaperone Ydj1 to enhance its stability and partitioning during asymmetric cell division and aging in yeast.PLoS biology · 2026Article
- Diatoms: harnessing nature's microscopic marvels for biosensing and multifaceted applications.Biophysical reviews · 2025Review
- Quantitative Characterization of Gene Regulatory Circuits Associated With Fungal Secondary Metabolism to Discover Novel Natural Products.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The de novo design and synthesis of yeast chromosome XIII facilitates investigations on aging.Nature communications · 2024Article
- Study of impacts of two types of cellular aging on the yeast bud morphogenesis.PLoS computational biology · 2024Article
- Millifluidic magnetophoresis-based chip for age-specific fractionation: evaluating the impact of age on metabolomics and gene expression in yeast.Lab on a chip · 2024Article
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- Measuring Replicative Lifespan in Cryptococcus neoformans.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Sis2 regulates yeast replicative lifespan in a dose-dependent manner.Nature communications · 2023Article
- Dietary change without caloric restriction maintains a youthful profile in ageing yeast.PLoS biology · 2023Article
- Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle.Nature metabolism · 2023Article
- Brain organoid-on-a-chip: A next-generation human brain avatar for recapitulating human brain physiology and pathology.Biomicrofluidics · 2022Article
- Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.FEMS microbiology reviews · 2022Review
- DetecDiv, a generalist deep-learning platform for automated cell division tracking and survival analysis.eLife · 2022Article
- High-resolution mass measurements of single budding yeast reveal linear growth segments.Nature communications · 2022Article
- Live cell microscopy: From image to insight.Biophysics reviews · 2022Review
51 more citing papers are in PubMed but not listed here.
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Important insights into aging have been generated with the genetically tractable and short-lived budding yeast. However, it is still impossible today to continuously track cells by high-resolution microscopic imaging (e.g., fluorescent imaging) throughout their entire lifespan. Instead, the field still needs to rely on a 50-y-old laborious and time-consuming method to assess the lifespan of yeast cells and to isolate differentially aged cells for microscopic snapshots via manual dissection of daughter cells from the larger mother cell. Here, we are unique in achieving continuous and high-resolution microscopic imaging of the entire replicative lifespan of single yeast cells. Our microfluidic dissection platform features an optically prealigned single focal plane and an integrated array of soft elastomer-based micropads, used together to allow for trapping of mother cells, removal of daughter cells, monitoring gradual changes in aging, and unprecedented microscopic imaging of the whole aging process. Using the platform, we found remarkable age-associated changes in phenotypes (e.g., that cells can show strikingly differential cell and vacuole morphologies at the moment of their deaths), indicating substantial heterogeneity in cell aging and death. We envision the microfluidic dissection platform to become a major tool in aging research.
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