ArticleMalaria journal2012
Extracellular ATP triggers proteolysis and cytosolic Ca²⁺ rise in Plasmodium berghei and Plasmodium yoelii malaria parasites.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 34 citations in OpenAlex.
- Evidence of G-Protein-Coupled Receptors (GPCR) in the Parasitic ProtozoaInternational journal of molecular sciences · 2021Review
- The Knockout for G Protein-Coupled Receptor-Like PfSR25 Increases the Susceptibility of Malaria Parasites to the Antimalarials Lumefantrine and Piperaquine but Not to Medicine for Malaria Venture Compounds.Frontiers in microbiology · 2021Article
- Review
- Differential expression pattern of co-inhibitory molecules on CD4Scientific reports · 2018Article
- Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell.Scientific reports · 2018Article
- Ned-19 inhibition of parasite growth and multiplication suggests a role for NAADP mediated signalling in the asexual development of Plasmodium falciparum.Malaria journal · 2017Article
- Plasmodium falciparum GFP-E-NTPDase expression at the intraerythrocytic stages and its inhibition blocks the development of the human malaria parasite.Purinergic signalling · 2017Article
- Plasmodium falciparum GPCR-like receptor SR25 mediates extracellular KScientific reports · 2017Article
- The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats.Journal of orthopaedic surgery and research · 2016Article
- Tumor necrosis factor reduces Plasmodium falciparum growth and activates calcium signaling in human malaria parasites.Biochimica et biophysica acta · 2016Article
- High-throughput matrix screening identifies synergistic and antagonistic antimalarial drug combinations.Scientific reports · 2015Article
- P2X and P2Y receptor signaling in red blood cells.Frontiers in molecular biosciences · 2015Review
- Cytoplasmic free Ca2+ is essential for multiple steps in malaria parasite egress from infected erythrocytes.Malaria journal · 2013Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundPlasmodium has a complex cell biology and it is essential to dissect the cell-signalling pathways underlying its survival within the host.
methodsUsing the fluorescence resonance energy transfer (FRET) peptide substrate Abz-AIKFFARQ-EDDnp and Fluo4/AM, the effects of extracellular ATP on triggering proteolysis and Ca²⁺ signalling in Plasmodium berghei and Plasmodium yoelii malaria parasites were investigated.
resultsThe protease activity was blocked in the presence of the purinergic receptor blockers suramin (50 μM) and PPADS (50 μM) or the extracellular and intracellular calcium chelators EGTA (5 mM) and BAPTA/AM (25, 100, 200 and 500 μM), respectively for P. yoelii and P. berghei. Addition of ATP (50, 70, 200 and 250 μM) to isolated parasites previously loaded with Fluo4/AM in a Ca²⁺-containing medium led to an increase in cytosolic calcium. This rise was blocked by pre-incubating the parasites with either purinergic antagonists PPADS (50 μM), TNP-ATP (50 μM) or the purinergic blockers KN-62 (10 μM) and Ip5I (10 μM). Incubating P. berghei infected cells with KN-62 (200 μM) resulted in a changed profile of merozoite surface protein 1 (MSP1) processing as revealed by western blot assays. Moreover incubating P. berghei for 17 h with KN-62 (10 μM) led to an increase in rings forms (82% ± 4, n = 11) and a decrease in trophozoite forms (18% ± 4, n = 11).
conclusionsThe data clearly show that purinergic signalling modulates P. berghei protease(s) activity and that MSP1 is one target in this pathway.
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