KLF6SV1 siRNA inhibits proliferation of human lens epithelial cells.

Ying Su, Yixin Qu, Chenggong Jiang, Lijuan Liu, Yanchun Shan, Feng Wang

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In one paragraph

Article in Molecular vision, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Ying SuDepartment of Ophthalmology, First clinical college of Harbin Medical University, Harbin, China.

Yixin Qu

Chenggong Jiang

Lijuan Liu

Yanchun Shan

Feng Wang

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo investigate whether transfection with Krüppel-like factor 6 splice variant 1 (KLF6SV1) siRNA can inhibit proliferation of human lens epithelial cell (HLEC).

methodsPlasmid containing KLF6SV1 siRNA was used to decrease the level of KLF6SV1 protein in HLEC. The expression of protein27 kinase inhibition protein 1 (p27(kip1)) and proliferation cell nuclear antigen (PCNA) was tested with western blot. Cell proliferation was assayed by 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazoliumbromide (MTT) assay and bromodeoxyuridine (BrdU) incorporation.

resultsKLF6SV1 siRNA can decrease KLF6SV1 expression which leads to increased levels of p27(kip1) and decreased expression of PCNA in HLEC. Cells transfected with pKLF6SV1 siRNA showed less viability compared with the control group in vitro.

conclusionsKLF6SV1 siRNA can effectively inhibit HLEC proliferation. It can be regarded as a novel target to treat posterior capsular opacity (PCO).

Indexed as

Cell ProliferationCells, CulturedCell SurvivalCyclin-Dependent Kinase Inhibitor p27Epithelial CellsGene ExpressionHumansKruppel-Like Factor 6Kruppel-Like Transcription FactorsLens, CrystallinePlasmidsProliferating Cell Nuclear AntigenProto-Oncogene ProteinsRNA, Small InterferingSignal TransductionTransfectionCDKN1B protein, humanCyclin-Dependent Kinase Inhibitor p27KLF6 protein, humanKruppel-Like Factor 6Kruppel-Like Transcription FactorsProliferating Cell Nuclear AntigenProto-Oncogene ProteinsRNA, Small Interfering

Identifiers

PMID22419853

PMCPMC3298452

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.