ArticleMolecular biology of the cell2012
Myosin-X functions in polarized epithelial cells.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Functional characterization of podocyte-expressed THSD7A in experimental membranous nephropathy.JCI insight · 2026Article
- Analysis of Tight Junction Formation and Epithelial Barrier Integrity by Calcium Switch and TEER Measurement.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Engineering a Human-Sized Common Bile Duct Prototype with Regenerative Potential: In Vitro Evaluation of Mechanics, Function, Degradation, and Immune Modulation.Advanced healthcare materials · 2025Article
- Hundreds of myosin 10s are pushed to the tips of filopodia and could cause traffic jams on actin.eLife · 2024Article
- Hundreds of myosin 10s are pushed to the tips of filopodia and could cause traffic jams on actin.bioRxiv : the preprint server for biology · 2024Article
- Intestinal Tuft Cells: Morphology, Function, and Implications for Human Health.Annual review of physiology · 2024Review
- Brain blood vessel autoantibodies in patients with NMDA and GABAFrontiers in cellular neuroscience · 2023Article
- MYO10 promotes transzonal projection-dependent germ line-somatic contact during mammalian folliculogenesis†.Biology of reproduction · 2022Article
- Cell fate determination and Hippo signaling pathway in preimplantation mouse embryo.Cell and tissue research · 2021Review
- Comparative analysis of the MyTH4-FERM myosins reveals insights into the determinants of actin track selection in polarized epithelia.Molecular biology of the cell · 2021Article
- Myosin-X is dispensable for spindle morphogenesis and positioning in the mouse oocyte.Development (Cambridge, England) · 2021Article
- Phenotypic analysis of Myo10 knockout (Myo10Scientific reports · 2019Article
- Myosin X is required for efficient melanoblast migration and melanoma initiation and metastasis.Scientific reports · 2018Article
- Myosin-10 independently influences mitotic spindle structure and mitotic progression.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Myosin MyTH4-FERM structures highlight important principles of convergent evolution.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Revisiting Myosin Families Through Large-scale Sequence Searches Leads to the Discovery of New Myosins.Evolutionary bioinformatics online · 2016Article
- Genetic Candidate Variants in Two Multigenerational Families with Childhood Apraxia of Speech.PloS one · 2016Article
- Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds.Developmental cell · 2015Article
- Myosin-X and disease.Experimental cell research · 2015Review
- Myosin X regulates neuronal radial migration through interacting with N-cadherin.Frontiers in cellular neuroscience · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Myosin-X (Myo10) is an unconventional myosin that localizes to the tips of filopodia and has critical functions in filopodia. Although Myo10 has been studied primarily in nonpolarized, fibroblast-like cells, Myo10 is expressed in vivo in many epithelia-rich tissues, such as kidney. In this study, we investigate the localization and functions of Myo10 in polarized epithelial cells, using Madin-Darby canine kidney II cells as a model system. Calcium-switch experiments demonstrate that, during junction assembly, green fluorescent protein-Myo10 localizes to lateral membrane cell-cell contacts and to filopodia-like structures imaged by total internal reflection fluorescence on the basal surface. Knockdown of Myo10 leads to delayed recruitment of E-cadherin and ZO-1 to junctions, as well as a delay in tight junction barrier formation, as indicated by a delay in the development of peak transepithelial electrical resistance (TER). Although Myo10 knockdown cells eventually mature into monolayers with normal TER, these monolayers do exhibit increased paracellular permeability to fluorescent dextrans. Importantly, knockdown of Myo10 leads to mitotic spindle misorientation, and in three-dimensional culture, Myo10 knockdown cysts exhibit defects in lumen formation. Together these results reveal that Myo10 functions in polarized epithelial cells in junction formation, regulation of paracellular permeability, and epithelial morphogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.