ArticleAnnals of botany2012
New chloroplast microsatellite markers suitable for assessing genetic diversity of Lolium perenne and other related grass species.
Article in Annals of botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 35 citations in OpenAlex.
- The Complete Chloroplast Genome ofGenes · 2022Article
- Taxonomical Evaluation of Plant Chloroplastic Markers by Bayesian Classifier.Frontiers in plant science · 2021Article
- Chloroplast Genome Sequencing, Comparative Analysis, and Discovery of Unique Cytoplasmic Variants in Pomegranate (Frontiers in genetics · 2021Article
- Evolutionary and phylogenetic aspects of the chloroplast genome of Chaenomeles species.Scientific reports · 2020Article
- Complete Chloroplast Genomes of 14 Mangroves: Phylogenetic and Comparative Genomic Analyses.BioMed research international · 2020Article
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- The Complete Plastome Sequences of ElevenGenes · 2018Article
- Chloroplast genome analyses and genomic resource development for epilithic sister genera Oresitrophe and Mukdenia (Saxifragaceae), using genome skimming data.BMC genomics · 2018Article
- Improving the Yield and Nutritional Quality of Forage Crops.Frontiers in plant science · 2018Review
- Development of Chloroplast Genomic Resources in Chinese YamBioMed research international · 2018Article
- Characterization of the whole chloroplast genome of Chikusichloa mutica and its comparison with other rice tribe (Oryzeae) species.PloS one · 2017Article
- Evaluation of six candidate DNA barcode loci for identification of five important invasive grasses in eastern Australia.PloS one · 2017Article
- Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.).Applications in plant sciences · 2016Article
- A comprehensive characterization of simple sequence repeats in pepper genomes provides valuable resources for marker development in Capsicum.Scientific reports · 2016Article
- Revealing genetic diversity of tree peonies at micro-evolution level with hyper-variable chloroplast markers and floral traits.Plant cell reports · 2012Article
- Breeding strategies for forage and grass improvement.Annals of botany · 2012Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsLolium perenne (perennial ryegrass) is the most important forage grass species of temperate regions. We have previously released the chloroplast genome sequence of L. perenne 'Cashel'. Here nine chloroplast microsatellite markers are published, which were designed based on knowledge about genetically variable regions within the L. perenne chloroplast genome. These markers were successfully used for characterizing the genetic diversity in Lolium and different grass species.
methodsChloroplast genomes of 14 Poaceae taxa were screened for mononucleotide microsatellite repeat regions and primers designed for their amplification from nine loci. The potential of these markers to assess genetic diversity was evaluated on a set of 16 Irish and 15 European L. perenne ecotypes, nine L. perenne cultivars, other Lolium taxa and other grass species. KEY
resultsAll analysed Poaceae chloroplast genomes contained more than 200 mononucleotide repeats (chloroplast simple sequence repeats, cpSSRs) of at least 7 bp in length, concentrated mainly in the large single copy region of the genome. Nucleotide composition varied considerably among subfamilies (with Pooideae biased towards poly A repeats). The nine new markers distinguish L. perenne from all non-Lolium taxa. TeaCpSSR28 was able to distinguish between all Lolium species and Lolium multiflorum due to an elongation of an A(8) mononucleotide repeat in L. multiflorum. TeaCpSSR31 detected a considerable degree of microsatellite length variation and single nucleotide polymorphism. TeaCpSSR27 revealed variation within some L. perenne accessions due to a 44-bp indel and was hence readily detected by simple agarose gel electrophoresis. Smaller insertion/deletion events or single nucleotide polymorphisms detected by these new markers could be visualized by polyacrylamide gel electrophoresis or DNA sequencing, respectively.
conclusionsThe new markers are a valuable tool for plant breeding companies, seed testing agencies and the wider scientific community due to their ability to monitor genetic diversity within breeding pools, to trace maternal inheritance and to distinguish closely related species.
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