ArticleThe Journal of biological chemistry2012
Novel basic protein, PfN23, functions as key macromolecule during nacre formation.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Egg capsule mineralization via vaterite transportation in the invasive apple snail Pomacea canaliculata.Molecular biology and evolution · 2026Article
- Exosome-Secreted Tropomyosin and Gigasin-6 Roles in Biomineralization Divergence Between Estuarine and Coastal Oysters.Marine biotechnology (New York, N.Y.) · 2026Article
- Nacre and Nacre-Inspired Materials: Historical Background, Definition, Fabrication Techniques and Gaps.Biomimetics (Basel, Switzerland) · 2026Review
- PU14, a Novel Matrix Protein, Participates in Pearl Oyster, Pinctada Fucata, Shell Formation.Marine biotechnology (New York, N.Y.) · 2021Article
- Novel globular C1q domain-containing protein (PmC1qDC-1) participates in shell formation and responses to pathogen-associated molecular patterns stimulation in Pinctada fucata martensii.Scientific reports · 2021Article
- Molecular mechanisms of biomineralization in marine invertebrates.The Journal of experimental biology · 2020Review
- Computationally predicted gene regulatory networks in molluscan biomineralization identify extracellular matrix production and ion transportation pathways.Bioinformatics (Oxford, England) · 2020Article
- Identification and Characterization of microRNAs and Their Predicted Functions in Biomineralization in the Pearl Oyster (Biology · 2019Article
- A basic protein, N25, from a mollusk modifies calcium carbonate morphology and shell biomineralization.The Journal of biological chemistry · 2019Article
- Transcriptional regulation of the matrix protein Shematrin-2 during shell formation in pearl oyster.The Journal of biological chemistry · 2018Article
- Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of Pinctada fucata.Scientific reports · 2018Article
- Novel Isoforms of N16 and N19 Families Implicated for the Nacreous Layer Formation in the Pearl Oyster Pinctada fucata.Marine biotechnology (New York, N.Y.) · 2018Article
- Amyloid Assembly Endows Gad m 1 with Biomineralization Properties.Biomolecules · 2018Article
- fam20C participates in the shell formation in the pearl oyster, Pinctada fucata.Scientific reports · 2018Article
- A Novel Matrix Protein, PfY2, Functions as a Crucial Macromolecule during Shell Formation.Scientific reports · 2017Article
- Identification and Characterization of the Lysine-Rich Matrix Protein Family in Pinctada fucata: Indicative of Roles in Shell Formation.Marine biotechnology (New York, N.Y.) · 2016Article
- Study of Binding Interaction between Pif80 Protein Fragment and Aragonite.Scientific reports · 2016Article
- Temporal and spatial expression patterns of biomineralization proteins during early development in the stony coral Pocillopora damicornis.Proceedings. Biological sciences · 2016Article
- Heterogeneous distribution of dye-labelled biomineralizaiton proteins in calcite crystals.Scientific reports · 2015Article
- In-depth proteomic analysis of shell matrix proteins of Pinctada fucata.Scientific reports · 2015Article
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9 authors.
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Abstract
The fine microstructure of nacre (mother of pearl) illustrates the beauty of nature. Proteins found in nacre were believed to be "natural hands" that control nacre formation. In the classical view of nacre formation, nucleation of the main minerals, calcium carbonate, is induced on and by the acidic proteins in nacre. However, the basic proteins were not expected to be components of nacre. Here, we reported that a novel basic protein, PfN23, was a key accelerator in the control over crystal growth in nacre. The expression profile, in situ immunostaining, and in vitro immunodetection assays showed that PfN23 was localized within calcium carbonate crystals in the nacre. Knocking down the expression of PfN23 in adults via double-stranded RNA injection led to a disordered nacre surface in adults. Blocking the translation of PfN23 in embryos using morpholino oligomers led to the arrest of larval development. The in vitro crystallization assay showed that PfN23 increases the rate of calcium carbonate deposition and induced the formation of aragonite crystals with characteristics close to nacre. In addition, we constructed the peptides and truncations of different regions of this protein and found that the positively charged C-terminal region was a key region for the function of PfN23 Taken together, the basic protein PfN23 may be a key accelerator in the control of crystal growth in nacre. This provides a valuable balance to the classic view that acidic proteins control calcium carbonate deposition in nacre.
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