ArticleThe Journal of biological chemistry2012
Vinculin functions as regulator of chondrogenesis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Role of external forces in the mechanobiology of stem and differentiated chondrogenic cells embedded in a tissue-engineered construct for cartilage repair.Journal of biomedical science · 2026Review
- Proteomic analysis reveals biomarkers associated with performance-based joint function and patient-reported outcomes in knee osteoarthritis.Osteoarthritis and cartilage open · 2025Article
- Ligand Composition and Coating Density Co-Modulate the Chondrocyte Function on Poly(glycerol-dodecanedioate).Journal of functional biomaterials · 2023Article
- Ion elemental-optimized layered double hydroxide nanoparticles promote chondrogenic differentiation and intervertebral disc regeneration of mesenchymal stem cells through focal adhesion signaling pathway.Bioactive materials · 2023Article
- Roles of focal adhesion proteins in skeleton and diseases.Acta pharmaceutica Sinica. B · 2023Review
- Protein tyrosine phosphatases in skeletal development and diseases.Bone research · 2022Review
- Kindlin-3 mutation in mesenchymal stem cells results in enhanced chondrogenesis.Experimental cell research · 2021Article
- Expression Profiling and Functional Analysis of CandidateFrontiers in genetics · 2021Article
- A novel glucosamine derivative with low cytotoxicity enhances chondrogenic differentiation of ATDC5.Journal of materials science. Materials in medicine · 2017Article
- Changes in Ultrastructure and Cytoskeletal Aspects of Human Normal and Osteoarthritic Chondrocytes Exposed to Interleukin-1β and Cyclical Hydrostatic Pressure.International journal of molecular sciences · 2015Article
- The importance of foetal movement for co-ordinated cartilage and bone development in utero : clinical consequences and potential for therapy.Bone & joint research · 2015Review
- Current understanding on the molecular basis of chondrogenesis.Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology · 2014Review
- Regulatory mechanisms for the development of growth plate cartilage.Cellular and molecular life sciences : CMLS · 2013Review
- Mechanobiology of human pluripotent stem cells.Tissue engineering. Part B, Reviews · 2013Review
- Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels.Acta biomaterialia · 2013Article
- FGF23 suppresses chondrocyte proliferation in the presence of soluble α-Klotho both in vitro and in vivo.The Journal of biological chemistry · 2013Article
- Mouse p63 variants and chondrogenesis.International journal of clinical and experimental pathology · 2013Article
- A network-based approach for predicting Hsp27 knock-out targets in mouse skeletal muscles.Computational and structural biotechnology journal · 2013Article
- A Critical Kinase Cascade in Neurological Disorders: PI 3-K, Akt, and mTOR.Future neurology · 2012Article
- Oxidant stress and signal transduction in the nervous system with the PI 3-K, Akt, and mTOR cascade.International journal of molecular sciences · 2012Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify the genes involved in chondrocytic differentiation, we applied gene trap mutagenesis to a murine mesenchymal chondrogenic cell line ATDC5 and isolated a clone in which the gene encoding vinculin was trapped. The trapped allele was assumed to express a fusion protein containing a truncated vinculin lacking the tail domain and the geo product derived from the trap vector. The truncated vinculin was suggested to exert a dominant negative effect. Impaired functioning of vinculin caused by gene trapping in ATDC5 cells or knockdown in primary chondrocytes resulted in the reduced expression of chondrocyte-specific genes, including Col2a1, aggrecan, and Col10a1. The expression of Runx2 also was suppressed by the dysfunctional vinculin. On the other hand, the expression of Sox9, encoding a key transcription factor for chondrogenesis, was retained. Knockdown of vinculin in metatarsal organ cultures impaired the growth of the explants and reduced the expression of Col2a1 and aggrecan. Gene trapping or knockdown of vinculin decreased the phosphorylation of ERK1/2 but increased that of Src homology 2 domain-containing tyrosine phosphatase 2 (SHP2) and Akt during chondrocytic differentiation, suggesting a disturbance of signaling by insulin-like growth factor I (IGF-I). Knockdown of vinculin in the metatarsal organ culture abrogated the IGF-I-induced growth and inhibited the up-regulation of Col2a1 and aggrecan expression by IGF-I. Loss of vinculin function in differentiating chondrocytes impaired the activation of the p38 MAPK pathway also, suggesting its involvement in the regulation of chondrogenesis by vinculin. Our results indicate a tissue-specific function of vinculin in cartilage whereby it controls chondrocytic differentiation.
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