ReviewBioEssays : news and reviews in molecular, cellular and developmental biology2012
Image analysis in fluorescence microscopy: bacterial dynamics as a case study.
Review in BioEssays : news and reviews in molecular, cellular and developmental biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Effect of shear rate on earlyBiofilm · 2024Article
- Quantification of C. neoformans Capsule Diameter.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Findaureus: An open-source application for locating Staphylococcus aureus in fluorescence-labelled infected bone tissue slices.PloS one · 2024Article
- Double-Labeling Method for Visualization and Quantification of Membrane-Associated Proteins inInternational journal of molecular sciences · 2023Article
- Optimization and Fabrication of Multi-Level Microchannels for Long-Term Imaging of Bacterial Growth and Expansion.Micromachines · 2022Article
- Advances and opportunities in image analysis of bacterial cells and communities.FEMS microbiology reviews · 2021Article
- Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans.Journal of visualized experiments : JoVE · 2018Article
- Application of Microfluidics in Experimental Ecology: The Importance of Being Spatial.Frontiers in microbiology · 2018Review
- Probing bacterial cell biology using image cytometry.Molecular microbiology · 2017Article
- Shedding light on biology of bacterial cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- A Tool for Alignment and Averaging of Sparse Fluorescence Signals in Rod-Shaped Bacteria.Biophysical journal · 2016Article
- How do bacteria localize proteins to the cell pole?Journal of cell science · 2014Review
- The price of tags in protein localization studies.Journal of bacteriology · 2012Article
- Förster resonance energy transfer (FRET) as a tool for dissecting the molecular mechanisms for maturation of the Shigella type III secretion needle tip complex.International journal of molecular sciences · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Fluorescence microscopy is the primary tool for studying complex processes inside individual living cells. Technical advances in both molecular biology and microscopy have made it possible to image cells from many genetic and environmental backgrounds. These images contain a vast amount of information, which is often hidden behind various sources of noise, convoluted with other information and stochastic in nature. Accessing the desired biological information therefore requires new tools of computational image analysis and modeling. Here, we review some of the recent advances in computational analysis of images obtained from fluorescence microscopy, focusing on bacterial systems. We emphasize techniques that are readily available to molecular and cell biologists but also point out examples where problem-specific image analyses are necessary. Thus, image analysis is not only a toolkit to be applied to new images but also an integral part of the design and implementation of a microscopy experiment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.