ArticleClinical epigenetics2012
Reproducibility and intraindividual variation over days in buccal cell DNA methylation of two asthma genes, interferon γ (IFNγ) and inducible nitric oxide synthase (iNOS).
Article in Clinical epigenetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Epigenetic signatures of asthma: a comprehensive study of DNA methylation and clinical markers.Clinical epigenetics · 2024Article
- Upper Airway Alarmin Cytokine Expression in Asthma of Different Severities.Journal of clinical medicine · 2024Article
- DNA Methylation and Allelic Polymorphism at the Dopamine Transporter Promoter Affect Internalizing and Externalizing Symptoms in Preschoolers.Child psychiatry and human development · 2021Article
- A review of epigenetic changes in asthma: methylation and acetylation.Clinical epigenetics · 2021Review
- Prospects for Use of Single-Cell Sequencing to Assess DNA Methylation in Asthma.Medical science monitor : international medical journal of experimental and clinical research · 2020Review
- Review
- Children's DAT1 Polymorphism Moderates the Relationship Between Parents' Psychological Profiles, Children's DAT Methylation, and Their Emotional/Behavioral Functioning in a Normative Sample.International journal of environmental research and public health · 2019Article
- Myeloid-derived suppressor cells (MDSCs) and mechanistic target of rapamycin (mTOR) signaling pathway interact through inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in asthma.American journal of translational research · 2019Article
- Article
- Short-term exposure to PMRespiratory research · 2017Article
- Effect of personal exposure to black carbon on changes in allergic asthma gene methylation measured 5 days later in urban children: importance of allergic sensitization.Clinical epigenetics · 2017Article
- Physical activity, black carbon exposure, and DNA methylation in theClinical epigenetics · 2017Article
- DNA Methylation at the DAT Promoter and Risk for Psychopathology: Intergenerational Transmission between School-Age Youths and Their Parents in a Community Sample.Frontiers in psychiatry · 2017Article
- Type I interferons exert anti-tumor effect via reversing immunosuppression mediated by mesenchymal stromal cells.Oncogene · 2016Article
- Smoking-Associated Site-Specific Differential Methylation in Buccal Mucosa in the COPDGene Study.American journal of respiratory cell and molecular biology · 2015Article
- Inducible nitric oxide synthase gene methylation and parkinsonism in manganese-exposed welders.Parkinsonism & related disorders · 2015Article
- Differences in DNA methylation profile of Th1 and Th2 cytokine genes are associated with tolerance acquisition in children with IgE-mediated cow's milk allergy.Clinical epigenetics · 2015Article
- 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome.Clinical epigenetics · 2014Article
- Allergic asthma biomarkers using systems approaches.Frontiers in genetics · 2014Review
- DNA methylation of the allergy regulatory gene interferon gamma varies by age, sex, and tissue type in asthmatics.Clinical epigenetics · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The biological mechanisms responsible for the onset and exacerbation of asthma symptoms in children may involve the epigenetic regulation of inflammatory genes after environmental exposures. Using buccal cells, we hypothesized that DNA methylation in promoter regions of two asthma genes, inducible nitric oxide synthase (iNOS) and interferon γ (IFNγ), can vary over several days. Repeat buccal samples were collected 4 to 7 days apart from 34 children participating in the Columbia Center for Children's Environmental Health (CCCEH) birth cohort study. Several field duplicates (sequential collection of two samples in the field) and replicates (one sample pyrosequenced twice) also were collected to ensure consistency with collection and laboratory procedures. DNA methylation was assessed by pyrosequencing a PCR of bisulfite-treated DNA. We found that replicate and field duplicate samples were correlated strongly (r = 0.86 to 0.99, P < 0.05), while repeat samples demonstrated low within-subject correlations (r = 0.19 to 0.56, P = 0.06 to 0.30). Our data reveal DNA methylation as a dynamic epigenetic mechanism that can be accessed safely and reproducibly in an inner city pediatric cohort using non-invasive buccal swabs and pyrosequencing technology.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.