ArticleAnalytical chemistry2012
Biopolymer system for cell recovery from microfluidic cell capture devices.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 90 citations in OpenAlex.
- Optimal Parameter Configuration of a Microfluidic Chip for High-Throughput, Label-Free Circulating Tumor Cell Separation and Enrichment Based on Inertial Focusing.Diagnostics (Basel, Switzerland) · 2023Article
- Aryl-diazonium salts offer a rapid and cost-efficient method to functionalize plastic microfluidic devices for increased immunoaffinity capture.Advanced materials technologies · 2023Article
- Review
- Flow Rate-Independent Multiscale Liquid Biopsy for Precision Oncology.ACS sensors · 2023Article
- Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics.Polymers · 2022Article
- Microfluidic Systems for Cancer Diagnosis and Applications.Micromachines · 2021Review
- Nanotechnology-Assisted Isolation and Analysis of Circulating Tumor Cells on Microfluidic Devices.Micromachines · 2020Review
- Past, Present, and Future of Affinity-based Cell Separation Technologies.Acta biomaterialia · 2020Review
- Efficient release of immunocaptured cells using coiled-coils in a microfluidic device.RSC advances · 2019Article
- Spatial presentation of biological molecules to cells by localized diffusive transfer.Lab on a chip · 2019Article
- Immunofunctional photodegradable poly(ethylene glycol) hydrogel surfaces for the capture and release of rare cells.Colloids and surfaces. B, Biointerfaces · 2019Article
- Emerging Technologies for Cancer Research: Towards Personalized Medicine with Microfluidic Platforms and 3D Tumor Models.Current medicinal chemistry · 2018Review
- Materials and microfluidics: enabling the efficient isolation and analysis of circulating tumour cells.Chemical Society reviews · 2017Review
- An integrated microfluidic system for the isolation and detection of ovarian circulating tumor cells using cell selection and enrichment methods.Biomicrofluidics · 2017Article
- Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip.Journal of the American Chemical Society · 2017Article
- Polymeric Biomaterials: Diverse Functions Enabled by Advances in Macromolecular Chemistry.Macromolecules · 2017Article
- Microfluidic techniques for high throughput single cell analysis.Current opinion in biotechnology · 2016Review
- Optimizing the Detection of Circulating Markers to Aid in Early Lung Cancer Detection.Cancers · 2016Review
- The Role of Surface Receptor Density in Surface-Initiated Polymerizations for Cancer Cell Isolation.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Tunable Thermal-Sensitive Polymer-Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells.Advanced materials (Deerfield Beach, Fla.) · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Microfluidic systems for affinity-based cell isolation have emerged as a promising approach for the isolation of specific cells from complex matrices (i.e., circulating tumor cells in whole blood). However, these technologies remain limited by the lack of reliable methods for the innocuous recovery of surface captured cells. Here, we present a biofunctional sacrificial hydrogel coating for microfluidic chips that enables the highly efficient release of isolated cells (99% ± 1%) following gel dissolution. This covalently cross-linked alginate biopolymer system is stable in a wide variety of physiologic solutions (including EDTA treated whole blood) and may be rapidly degraded via backbone cleavage with alginate lyase. The capture and release of EpCAM expressing cancer cells using this approach was found to have no significant effect on cell viability or proliferative potential, and recovered cells were demonstrated to be compatible with downstream immunostaining and FISH analysis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.