ArticlePloS one2012
Cigarette smoke upregulates rat coronary artery endothelin receptors in vivo.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Effects of moderate-intensity aerobic exercise on coronary artery structure and function in type 2 diabetic rats.Scientific reports · 2026Article
- Connexins may play a critical role in cigarette smoke-induced pulmonary hypertension.Archives of toxicology · 2022Review
- Nicotine and vascular dysfunction.Acta physiologica (Oxford, England) · 2021Review
- The Smoking Paradox: A Twist in the Tale of Vasospastic Angina.Journal of vascular medicine & surgery · 2021Article
- Quercetin attenuates reduced uterine perfusion pressure -induced hypertension in pregnant rats through regulation of endothelin-1 and endothelin-1 type A receptor.Lipids in health and disease · 2020Article
- Genetic susceptibility of five tagSNPs in the endothelin-1 (Bioscience reports · 2018Article
- Air Pollution-Induced Vascular Dysfunction: Potential Role of Endothelin-1 (ET-1) System.Cardiovascular toxicology · 2016Review
- Effect of Shenkang granules on the progression of chronic renal failure in 5/6 nephrectomized rats.Experimental and therapeutic medicine · 2015Article
- Smoking and choroidal thickness in patients over 65 with early-atrophic age-related macular degeneration and normals.Eye (London, England) · 2014Observational
- The effect of cigarette smoke exposure on spinal cord injury in rats.Journal of neurotrauma · 2013Article
- Altered endothelin receptor expression and affinity in spontaneously hypertensive rat cerebral and coronary arteries.PloS one · 2013Article
- Minimally modified LDL upregulates endothelin type A receptors in rat coronary arterial smooth muscle cells.Mediators of inflammation · 2013Article
- Article
- Evaluation of Foveal Avascular Zone and Capillary Plexus in Smokers Using Optical Coherence Tomography Angiography.Journal of current ophthalmologyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCigarette smoking is a strong cardiovascular risk factor and endothelin (ET) receptors are related to coronary artery diseases. The present study established an in vivo secondhand smoke (SHS) exposure model and investigated the hypothesis that cigarette smoke induces ET receptor upregulation in rat coronary arteries and its possible underlying mechanisms. METHODOLOGY/PRINCIPAL
findingsRats were exposed to SHS for 200 min daily for 8 weeks. The coronary arteries were isolated and examined. The vasoconstriction was studied by a sensitive myograph. The expression of mRNA and protein for receptors was examined by real-time PCR, Western blot and immunofluorescence. Compared to fresh air exposure, SHS increased contractile responses mediated by endothelin type A (ET(A)) and type B (ET(B)) receptors in coronary arteries. In parallel, the expression of mRNA and protein for ET(A) and ET(B) receptors of smoke exposed rats were higher than that of animals exposed to fresh air, suggesting that SHS upregulates ET(A) and ET(B) receptors in coronary arteries in vivo. Immunofluorescence staining showed that the enhanced receptor expression was localized to the smooth muscle cells of coronary arteries. The protein levels of phosphorylated (p)-Raf-1 and p-ERK1/2 in smoke exposed rats were significantly higher than in control rats, demonstrating that SHS induces the activation of the Raf/ERK/MAPK pathway. Treatment with Raf-1 inhibitor GW5074 suppressed SHS-induced enhanced contraction mediated by ET(A) receptors, and inhibited the elevated mRNA and protein levels of ET(A) and ET(B) receptors caused by SHS. The results of correlation and regression analysis showed that phosphorylation of Raf and ERK1/2 were independent determinants to affect protein expression of ET(B) and ET(A) receptors. CONCLUSIONS/SIGNIFICANCE: Cigarette smoke upregulates ET(B) and ET(A) receptors in rat coronary artery, which is associated with the activation of the Raf/ERK/MAPK pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.