ArticlePloS one2012
Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03924843 (Responsivity and Reproducibility of Messenger and Micro RNA Airway Inflammatory Markers - a Pilot Study), which is not on this map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Responsivity and Reproducibility of Messenger and Micro RNA Airway Inflammatory Markers - a Pilot Study
Who cites it
47 citing papers in PubMed, 83 citations in OpenAlex.
- Nasal Epithelial Organoids as Translational Platforms in Inflammatory, Infectious, and Precision Medicine Applications: A Systematic Review.Journal of clinical medicine · 2026Review
- Upper and Lower Respiratory Tract Compartmentalization in Pediatric Stem Cell Transplantation.American journal of respiratory cell and molecular biology · 2026Article
- The Role of Enoxaparin in Influenza Virus Infections and its Therapeutic Implications.The Journal of infectious diseases · 2026Article
- Comparative Effects of Cigarette Smoke and Heated Tobacco Product Aerosols on Biofilm Production by Respiratory Pathogens.Microorganisms · 2025Article
- Organotypic Airway Models Derived from Primary Human Nasal Epithelial Cells.Current allergy and asthma reports · 2025Review
- Upper and Lower Respiratory Tract Compartmentalization in Pediatric Stem Cell Transplantation.medRxiv : the preprint server for health sciences · 2025Article
- Limited response of primary nasal epithelial cells toMicrobiology spectrum · 2025Article
- Primary human nasal, nasopharyngeal, and bronchial epithelia show distinct immune responses to various pathogens.Pathogens and disease · 2025Article
- Pathogenic Cell in COPD: Mechanisms of Airway Remodeling, Immune Dysregulation, and Therapeutic Implications.International journal of chronic obstructive pulmonary disease · 2025Review
- Bronchoscopic biopsies - a novel source for primary airway epithelial cells in respiratory research.Respiratory research · 2024Article
- Upper Airway Alarmin Cytokine Expression in Asthma of Different Severities.Journal of clinical medicine · 2024Article
- Differential interferon responses to influenza A and B viruses in primary ferret respiratory epithelial cells.Journal of virology · 2024Article
- Genomic attributes of airway commensal bacteria and mucosa.Communications biology · 2024Article
- Culture expansion of primary human nasal epithelial cells (NEC) isolated with a nasal scraping spoon.The Journal of international medical research · 2023Observational
- Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions.Journal of visualized experiments : JoVE · 2023Article
- Infection of primary nasal epithelial cells differentiates among lethal and seasonal human coronaviruses.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nasal biomarkers of immune function differ based on smoking and respiratory disease status.Physiological reports · 2023Article
- Organotypic sinonasal airway culture systems are predictive of the mucociliary phenotype produced by bronchial airway epithelial cells.Scientific reports · 2022Article
- Infection of primary nasal epithelial cells differentiates among lethal and seasonal human coronaviruses.bioRxiv : the preprint server for biology · 2022Article
- Impact of inhaled pollutants on response to viral infection in controlled exposures.The Journal of allergy and clinical immunology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over immortalised cell lines. Nasal epithelial cells are easier to obtain than bronchial epithelial cells and can be used as an alternative for in vitro studies. Our objective was to compare nasal and bronchial epithelial cells from subjects with COPD to establish if these cells respond similarly to pro-inflammatory stimuli. Cell cultures from paired nasal and bronchial brushings (21 subjects) were incubated with cigarette smoke extract (CSE) prior to stimulation with Pseudomonas aeruginosa lipopolysaccharide. IL-6 and IL-8 were measured by ELISA and Toll-like receptor 4 (TLR-4) message and expression by RT-PCR and FACS respectively. IL-8 release correlated significantly between the two cell types. IL-6 secretion was significantly less from bronchial compared to nasal epithelial cells and secreted concentrations did not correlate. A 4 h CSE incubation was immunosuppressive for both nasal and bronchial cells, however prolonged incubation for 24 h was pro-inflammatory solely for the nasal cells. CSE reduced TLR-4 expression in bronchial cells only after 24 h, and was without effect on mRNA expression. In subjects with COPD, nasal epithelial cells cannot substitute for in vitro bronchial epithelial cells in airway inflammation studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.