ArticlePloS one2012
Rugged single domain antibody detection elements for Bacillus anthracis spores and vegetative cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy.Frontiers in cellular and infection microbiology · 2021Review
- Infectious disease antibodies for biomedical applications: A mini review of immune antibody phage library repertoire.International journal of biological macromolecules · 2020Review
- Rapid Microscopic Detection ofMicroorganisms · 2020Article
- Optimizing the Design of Diatom Biosilica-Targeted Fusion Proteins in Biosensor Construction forBiology · 2020Article
- Genetic Fusion of an Anti-BclA Single-Domain Antibody with Beta Galactosidase.Antibodies (Basel, Switzerland) · 2018Article
- Enhanced production and purification of recombinant surface array protein (Sap) for use in detection of3 Biotech · 2018Article
- Pairing Alpaca and Llama-Derived Single Domain Antibodies to Enhance Immunoassays for Ricin.Antibodies (Basel, Switzerland) · 2017Article
- Nanomaterial-based sensors for the detection of biological threat agents.Materials today (Kidlington, England) · 2016Review
- Recombinant antibodies for diagnostics and therapy against pathogens and toxins generated by phage display.Proteomics. Clinical applications · 2016Review
- Improving the biophysical properties of anti-ricin single-domain antibodies.Biotechnology reports (Amsterdam, Netherlands) · 2015Article
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- Article
- Development and evaluation of single domain antibodies for vaccinia and the L1 antigen.PloS one · 2014Article
- Immuno capture PCR for rapid and sensitive identification of pathogenic Bacillus anthracis.World journal of microbiology & biotechnology · 2013Article
- Contributions of the complementarity determining regions to the thermal stability of a single-domain antibody.PloS one · 2013Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Significant efforts to develop both laboratory and field-based detection assays for an array of potential biological threats started well before the anthrax attacks of 2001 and have continued with renewed urgency following. While numerous assays and methods have been explored that are suitable for laboratory utilization, detection in the field is often complicated by requirements for functionality in austere environments, where limited cold-chain facilities exist. In an effort to overcome these assay limitations for Bacillus anthracis, one of the most recognizable threats, a series of single domain antibodies (sdAbs) were isolated from a phage display library prepared from immunized llamas. Characterization of target specificity, affinity, and thermal stability was conducted for six sdAb families isolated from rounds of selection against the bacterial spore. The protein target for all six sdAb families was determined to be the S-layer protein EA1, which is present in both vegetative cells and bacterial spores. All of the sdAbs examined exhibited a high degree of specificity for the target bacterium and its spore, with affinities in the nanomolar range, and the ability to refold into functional antigen-binding molecules following several rounds of thermal denaturation and refolding. This research demonstrates the capabilities of these sdAbs and their potential for integration into current and developing assays and biosensors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.