ArticlePloS one2012
Direct interaction of the N-terminal domain of ribosomal protein S1 with protein S2 in Escherichia coli.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Structural insights into the binding of bS1 to the ribosome.Nucleic acids research · 2023Article
- Selective recruitment of stress-responsive mRNAs to ribosomes for translation by acetylated protein S1 during nutrient stress in Escherichia coli.Communications biology · 2022Article
- Interaction between Phage T4 Protein RIII and Host Ribosomal Protein S1 Inhibits Endoribonuclease RegB Activation.International journal of molecular sciences · 2022Article
- Structures of Mycobacterium smegmatis 70S ribosomes in complex with HPF, tmRNA, and P-tRNA.Scientific reports · 2018Article
- Leaderless mRNAs in the Spotlight: Ancient but Not Outdated!Microbiology spectrum · 2018Review
- Structural dynamics of protein S1 on the 70S ribosome visualized by ensemble cryo-EM.Methods (San Diego, Calif.) · 2018Article
- Transcription-translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits.Nucleic acids research · 2017Article
- Unique localization of the plastid-specific ribosomal proteins in the chloroplast ribosome small subunit provides mechanistic insights into the chloroplastic translation.Nucleic acids research · 2017Article
- Structural basis for RNA-genome recognition during bacteriophage Qβ replication.Nucleic acids research · 2015Article
- Ribosome. The structure of the human mitochondrial ribosome.Science (New York, N.Y.) · 2015Article
- Immunoproteomic identification of immunodominant antigens independent of the time of infection in Brucella abortus 2308-challenged cattle.Veterinary research · 2015Article
- Structural basis for the interaction of protein S1 with the Escherichia coli ribosome.Nucleic acids research · 2015Article
- Ribosomal proteins as unrevealed caretakers for cellular stress and genomic instability.Oncotarget · 2014Review
- Escherichia coli ribosomal protein S1 unfolds structured mRNAs onto the ribosome for active translation initiation.PLoS biology · 2013Article
- Ribosome heterogeneity: another level of complexity in bacterial translation regulation.Current opinion in microbiology · 2013Review
- A single missense mutation in a coiled-coil domain of Escherichia coli ribosomal protein S2 confers a thermosensitive phenotype that can be suppressed by ribosomal protein S1.Journal of bacteriology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite of the high resolution structure available for the E. coli ribosome, hitherto the structure and localization of the essential ribosomal protein S1 on the 30 S subunit still remains to be elucidated. It was previously reported that protein S1 binds to the ribosome via protein-protein interaction at the two N-terminal domains. Moreover, protein S2 was shown to be required for binding of protein S1 to the ribosome. Here, we present evidence that the N-terminal domain of S1 (amino acids 1-106; S1(106)) is necessary and sufficient for the interaction with protein S2 as well as for ribosome binding. We show that over production of protein S1(106) affects E. coli growth by displacing native protein S1 from its binding pocket on the ribosome. In addition, our data reveal that the coiled-coil domain of protein S2 (S2α(2)) is sufficient to allow protein S1 to bind to the ribosome. Taken together, these data uncover the crucial elements required for the S1/S2 interaction, which is pivotal for translation initiation on canonical mRNAs in gram-negative bacteria. The results are discussed in terms of a model wherein the S1/S2 interaction surface could represent a possible target to modulate the selectivity of the translational machinery and thereby alter the translational program under distinct conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.