ArticlePloS one2012
Evaluation of cervical mucosa in transmission bottleneck during acute HIV-1 infection using a cervical tissue-based organ culture.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Article
- Methodology for reliable and reproducible cryopreservation of human cervical tissue.Cryobiology · 2017Article
- Seminal plasma induces inflammation and enhances HIV-1 replication in human cervical tissue explants.PLoS pathogens · 2017Article
- Infection of ectocervical tissue and universal targeting of T-cells mediated by primary non-macrophage-tropic and highly macrophage-tropic HIV-1 R5 envelopes.Retrovirology · 2015Article
- Cellular superspreaders: an epidemiological perspective on HIV infection inside the body.PLoS pathogens · 2014Article
- Transmission and evolution of hepatitis C virus in HCV seroconverters in HIV infected subjects.Virology · 2014Article
- Antigenicity and immunogenicity of transmitted/founder, consensus, and chronic envelope glycoproteins of human immunodeficiency virus type 1.Journal of virology · 2013Article
- Use of human mucosal tissue to study HIV-1 pathogenesis and evaluate HIV-1 prevention modalities.Current HIV/AIDS reports · 2013Review
- Study of HIV-1 transmission across cervical mucosa to tonsil tissue cells using an organ culture.American journal of reproductive immunology (New York, N.Y. : 1989) · 2013Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundAlthough there are different strains of HIV-1 in a chronically infected individual, only one or limited virus strains are successfully transmitted to a new individual. The reason for this "transmission bottleneck" is as yet unknown. METHODOLOGY/PRINCIPAL
findingsA human cervical explant model was used to measure HIV-1 transmission efficiency of viral strains from chronic infections, and transmitter/founder variants. We also evaluated the genetic characteristics of HIV-1 variants in the inoculums compared to those transmitted across the cervical mucosa. Eight different HIV-1 isolates were used in this study, six chronic isolates and two transmitter/founder viruses. The transmission efficiency of the chronic and transmitter/founder virus isolates and the viral diversity of chronic isolates before and after viral transmission were assessed. The results indicate that transmitter/founder viruses did not display higher transmission efficiency than chronic HIV-1 isolates. Furthermore, no evidence for a difference in diversity was found between the inoculums and transmitted virus strains. Phylogenetic analysis indicated that the sequences of variants in the inoculums and those present in transmitted virus intermingled irrespective of co-receptor usage. In addition, the inoculum and transmitted variants had a similar pairwise distance distribution.
conclusionThere was no selection of a single or limited number of viral variants during HIV-1 transmission across the cervical mucosa in the organ culture model, indicating that the cervical mucosa alone may not produce the transmission bottleneck of HIV-1 infection observed in vivo.
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