ArticlePloS one2012
Transcriptomics comparison between porcine adipose and bone marrow mesenchymal stem cells during in vitro osteogenic and adipogenic differentiation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 76 citations in OpenAlex.
- Single-nucleus RNA sequencing and lipidomics reveal characteristics of transcriptional and lipid composition in porcine longissimus dorsi muscle.BMC genomics · 2024Article
- Effect of metabolically divergent pig breeds and tissues on mesenchymal stem cell expression patterns during adipogenesis.BMC genomics · 2024Article
- Deciphering transcriptome patterns in porcine mesenchymal stem cells promoting phenotypic maintenance and differentiation by key driver genes.Frontiers in cell and developmental biology · 2024Article
- Isolation and characterization of farm pig adipose tissue-derived mesenchymal stromal/stem cells.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2022Article
- RFX1: a promising therapeutic arsenal against cancer.Cancer cell international · 2021Review
- Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow-derived stromal cells.Journal of cellular and molecular medicine · 2020Article
- Prepartum dietary energy intake alters adipose tissue transcriptome profiles during the periparturient period in Holstein dairy cows.Journal of animal science and biotechnology · 2020Article
- The inclusion of zinc into mineralized collagen scaffolds for craniofacial bone repair applications.Acta biomaterialia · 2019Article
- Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis.Scientific reports · 2018Article
- Polymer-mineral scaffold augments in vivo equine multipotent stromal cell osteogenesis.Stem cell research & therapy · 2018Article
- MAPK signaling has stage-dependent osteogenic effects on human adipose-derived stem cells in vitro.Connective tissue research · 2018Article
- Adipose Tissue as a Strategic Source of Mesenchymal Stem Cells in Bone Regeneration: A Topical Review on the Most Promising Craniomaxillofacial Applications.International journal of molecular sciences · 2017Review
- Long non-coding RNA MEG3 inhibits adipogenesis and promotes osteogenesis of human adipose-derived mesenchymal stem cells via miR-140-5p.Molecular and cellular biochemistry · 2017Article
- Properties of porcine adipose-derived stem cells and their applications in preclinical models.Adipocyte · 2017Review
- Similarities and differences between porcine mandibular and limb bone marrow mesenchymal stem cells.Archives of oral biology · 2017Article
- Patterning Three-Dimensional Hydrogel Microenvironments Using Hyperbranched Polyglycerols for Independent Control of Mesh Size and Stiffness.Biomacromolecules · 2017Article
- MiR-26a functions oppositely in osteogenic differentiation of BMSCs and ADSCs depending on distinct activation and roles of Wnt and BMP signaling pathway.Cell death & disease · 2015Article
- Expression analysis of human adipose-derived stem cells during in vitro differentiation to an adipocyte lineage.BMC medical genomics · 2015Article
- Osteogenic performance of donor-matched human adipose and bone marrow mesenchymal cells under dynamic culture.Tissue engineering. Part A · 2015Article
- Impact of tissue-specific stem cells on lineage-specific differentiation: a focus on the musculoskeletal system.Stem cell reviews and reports · 2015Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Bone-marrow mesenchymal stem cells (BMSC) are considered the gold standard for use in tissue regeneration among mesenchymal stem cells (MSC). The abundance and ease of harvest make the adipose-derived stem cells (ASC) an attractive alternative to BMSC. The aim of the present study was to compare the transcriptome of ASC and BMSC, respectively isolated from subcutaneous adipose tissue and femur of 3 adult pigs, during in vitro osteogenic and adipogenic differentiation for up to four weeks. At 0, 2, 7, and 21 days of differentiation RNA was extracted for microarray analysis. A False Discovery Rate ≤0.05 for overall interactions effect and P<0.001 between comparisons were used to determine differentially expressed genes (DEG). Ingenuity Pathway Analysis and DAVID performed the functional analysis of the DEG. Functional analysis of highest expressed genes in MSC and genes more expressed in MSC vs. fully differentiated tissues indicated low immunity and high angiogenic capacity. Only 64 genes were differentially expressed between ASC and BMSC before differentiation. The functional analysis uncovered a potential larger angiogenic, osteogenic, migration, and neurogenic capacity in BMSC and myogenic capacity in ASC. Less than 200 DEG were uncovered between ASC and BMSC during differentiation. Functional analysis also revealed an overall greater lipid metabolism in ASC, while BMSC had a greater cell growth and proliferation. The time course transcriptomic comparison between differentiation types uncovered <500 DEG necessary to determine cell fate. The functional analysis indicated that osteogenesis had a larger cell proliferation and cytoskeleton organization with a crucial role of G-proteins. Adipogenesis was driven by PPAR signaling and had greater angiogenesis, lipid metabolism, migration, and tumorigenesis capacity. Overall the data indicated that the transcriptome of the two MSC is relatively similar across the conditions studied. In addition, functional analysis data might indicate differences in therapeutic application.
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Registered trials
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