ArticlePLoS genetics2012
Precocious metamorphosis in the juvenile hormone-deficient mutant of the silkworm, Bombyx mori.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 178 citations in OpenAlex.
- SV2B is a crucial factor for early larval development in the silkworm, Bombyx mori.Insect science · 2026Article
- Molecular insights into juvenile hormone maturation by juvenile hormone acid methyltransferase.The Journal of biological chemistry · 2026Article
- Two cytochrome P450 epoxidases mediate juvenile hormone biosynthesis in Drosophila melanogaster.Insect biochemistry and molecular biology · 2026Article
- Evolution of complete metamorphosis through temporal shifts in Chronologically inappropriate morphogenesis (Chinmo) and Broad.Development (Cambridge, England) · 2026Article
- Two cytochrome P450 epoxidases mediate juvenile hormone biosynthesis inbioRxiv : the preprint server for biology · 2025Article
- Resource limitation during larval growth leads to higher flight propensity in adult beetles.Biology letters · 2025Article
- Functional Annotation and Comparative Analysis of Cytochrome P450 Protein Family Genes in Nine Chironomidae Species.Biology · 2025Article
- Role of histone methylation in insect development: KMT5A regulates ecdysteroid biosynthesis during metamorphosis of Tribolium castaneum.Insect biochemistry and molecular biology · 2025Article
- Functional Study of the Role of theInternational journal of molecular sciences · 2024Article
- Cyp6g2 is the major P450 epoxidase responsible for juvenile hormone biosynthesis in Drosophila melanogaster.BMC biology · 2024Article
- SilkMeta: a comprehensive platform for sharing and exploiting pan-genomic and multi-omic silkworm data.Nucleic acids research · 2024Article
- DisruptingFrontiers in physiology · 2024Article
- Exorista sorbillans (Diptera: Tachinidae) parasitism shortens host larvae growth duration by regulating ecdysone and juvenile hormone titers in Bombyx mori (Lepidoptera: Bombycidae).Journal of insect science (Online) · 2023Article
- Insights into the Effects of Insecticides on Aphids (Hemiptera: Aphididae): Resistance Mechanisms and Molecular Basis.International journal of molecular sciences · 2023Review
- A caste differentiation mutant elucidates the evolution of socially parasitic ants.Current biology : CB · 2023Article
- Genome-Wide Identification and Expression Pattern of Cytochrome P450 Genes in the Social AphidInsects · 2023Article
- POU-M2 promotes juvenile hormone biosynthesis by directly activating the transcription of juvenile hormone synthetic enzyme genes inOpen biology · 2022Article
- Functional Characterization of Allatostatin C (PISCF/AST) and Juvenile Hormone Acid O-Methyltransferase inInternational journal of molecular sciences · 2022Article
- Epoxidation of juvenile hormone was a key innovation improving insect reproductive fitness.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Role of juvenile hormone receptorZoological research · 2021Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insect molting and metamorphosis are intricately governed by two hormones, ecdysteroids and juvenile hormones (JHs). JHs prevent precocious metamorphosis and allow the larva to undergo multiple rounds of molting until it attains the proper size for metamorphosis. In the silkworm, Bombyx mori, several "moltinism" mutations have been identified that exhibit variations in the number of larval molts; however, none of them have been characterized molecularly. Here we report the identification and characterization of the gene responsible for the dimolting (mod) mutant that undergoes precocious metamorphosis with fewer larval-larval molts. We show that the mod mutation results in complete loss of JHs in the larval hemolymph and that the mutant phenotype can be rescued by topical application of a JH analog. We performed positional cloning of mod and found a null mutation in the cytochrome P450 gene CYP15C1 in the mod allele. We also demonstrated that CYP15C1 is specifically expressed in the corpus allatum, an endocrine organ that synthesizes and secretes JHs. Furthermore, a biochemical experiment showed that CYP15C1 epoxidizes farnesoic acid to JH acid in a highly stereospecific manner. Precocious metamorphosis of mod larvae was rescued when the wild-type allele of CYP15C1 was expressed in transgenic mod larvae using the GAL4/UAS system. Our data therefore reveal that CYP15C1 is the gene responsible for the mod mutation and is essential for JH biosynthesis. Remarkably, precocious larval-pupal transition in mod larvae does not occur in the first or second instar, suggesting that authentic epoxidized JHs are not essential in very young larvae of B. mori. Our identification of a JH-deficient mutant in this model insect will lead to a greater understanding of the molecular basis of the hormonal control of development and metamorphosis.
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