ArticlePLoS biology2012
USF binding sequences from the HS4 insulator element impose early replication timing on a vertebrate replicator.
Article in PLoS biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Transcription elongation can be sufficient, but is not necessary, to advance replication timing.EMBO reports · 2026Article
- Transcription at an inducible common fragile site reveals replication origin strength hierarchy.Nucleic acids research · 2026Article
- Transcription can be sufficient, but is not necessary, to advance replication timing.bioRxiv : the preprint server for biology · 2025Article
- Review
- Dimeric G-quadruplex motifs-induced NFRs determine strong replication origins in vertebrates.Nature communications · 2023Article
- Replication timing and transcriptional control: beyond cause and effect - part IV.Current opinion in genetics & development · 2023Review
- Mammalian DNA Replication Timing.Cold Spring Harbor perspectives in biology · 2021Review
- Clustering of strong replicators associated with active promoters is sufficient to establish an early-replicating domain.The EMBO journal · 2020Article
- Control of DNA replication timing in the 3D genome.Nature reviews. Molecular cell biology · 2019Review
- Replication timing networks reveal a link between transcription regulatory circuits and replication timing control.Genome research · 2019Article
- Replication dynamics of individual loci in single living cells reveal changes in the degree of replication stochasticity through S phase.Nucleic acids research · 2019Article
- Review
- Article
- Transcription-dependent regulation of replication dynamics modulates genome stability.Nature structural & molecular biology · 2019Article
- Bacterial artificial chromosomes establish replication timing and sub-nuclear compartment de novo as extra-chromosomal vectors.Nucleic acids research · 2018Article
- The impact of replication stress on replication dynamics and DNA damage in vertebrate cells.Nature reviews. Genetics · 2017Review
- Regulation of Replication Origins.Advances in experimental medicine and biology · 2017Review
- DNA replication origins-where do we begin?Genes & development · 2016Review
- Replication timing and transcriptional control: beyond cause and effect-part III.Current opinion in cell biology · 2016Review
- Replication origins: determinants or consequences of nuclear organization?Current opinion in genetics & development · 2016Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The nuclear genomes of vertebrates show a highly organized program of DNA replication where GC-rich isochores are replicated early in S-phase, while AT-rich isochores are late replicating. GC-rich regions are gene dense and are enriched for active transcription, suggesting a connection between gene regulation and replication timing. Insulator elements can organize independent domains of gene transcription and are suitable candidates for being key regulators of replication timing. We have tested the impact of inserting a strong replication origin flanked by the β-globin HS4 insulator on the replication timing of naturally late replicating regions in two different avian cell types, DT40 (lymphoid) and 6C2 (erythroid). We find that the HS4 insulator has the capacity to impose a shift to earlier replication. This shift requires the presence of HS4 on both sides of the replication origin and results in an advance of replication timing of the target locus from the second half of S-phase to the first half when a transcribed gene is positioned nearby. Moreover, we find that the USF transcription factor binding site is the key cis-element inside the HS4 insulator that controls replication timing. Taken together, our data identify a combination of cis-elements that might constitute the basic unit of multi-replicon megabase-sized early domains of DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.