ArticleProceedings of the National Academy of Sciences of the United States of America2012
Lateral mobility of individual integrin nanoclusters orchestrates the onset for leukocyte adhesion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 101 citations in OpenAlex.
- Outside-In Signaling as a Detonator-like Stimulus of Integrin Activation by Promoting Both Affinity and Avidity.Biomolecules · 2026Review
- Platelet-derived integrin- and tetraspanin-enriched tethers exacerbate severe inflammation.Science (New York, N.Y.) · 2026Article
- Podosome Nucleation Is Facilitated by Multivalent Interactions between Syk and ITAM-containing Membrane Complexes.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Measuring Integrin Force Loading Rates Using a Two-Step DNA Tension Sensor.Journal of the American Chemical Society · 2024Article
- Hexagonal Plasmonic Arrays for High-Throughput Multicolor Single-Molecule Studies.ACS applied materials & interfaces · 2024Article
- Measuring integrin force loading rates using a two-step DNA tension sensor.bioRxiv : the preprint server for biology · 2024Article
- Quantitative imaging of vesicle-protein interactions reveals close cooperation among proteins.Journal of extracellular vesicles · 2023Article
- Mesoscience in cell biology and cancer research.Cancer innovation · 2022Review
- Article
- Stochastic particle unbinding modulates growth dynamics and size of transcription factor condensates in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Significance of Receptor Mobility in Multivalent Binding on Lipid Membranes.Angewandte Chemie (International ed. in English) · 2022Review
- Binding kinetics of liposome conjugated E-selectin and P-selectin glycoprotein ligand-1 measured with atomic force microscopy.Colloids and surfaces. B, Biointerfaces · 2021Article
- Shear forces induce ICAM-1 nanoclustering on endothelial cells that impact on T-cell migration.Biophysical journal · 2021Article
- LFA-1 cluster formation in T-cells depends on L-plastin phosphorylation regulated by P90Cellular and molecular life sciences : CMLS · 2021Article
- Integrin Regulation in Immunological and Cancerous Cells and Exosomes.International journal of molecular sciences · 2021Review
- Natural Killer Cell Integrins and Their Functions in Tissue Residency.Frontiers in immunology · 2021Review
- Neuraminidase-3 Is a Negative Regulator of LFA-1 Adhesion.Frontiers in chemistry · 2019Article
- LFA-1 in T Cell Migration and Differentiation.Frontiers in immunology · 2018Review
- Tetraspanin CD9: A Key Regulator of Cell Adhesion in the Immune System.Frontiers in immunology · 2018Review
- A DNA origami platform for quantifying protein copy number in super-resolution.Nature methods · 2017Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Integrins are cell membrane adhesion receptors involved in morphogenesis, immunity, tissue healing, and metastasis. A central, yet unresolved question regarding the function of integrins is how these receptors regulate both their conformation and dynamic nanoscale organization on the membrane to generate adhesion-competent microclusters upon ligand binding. Here we exploit the high spatial (nanometer) accuracy and temporal resolution of single-dye tracking to dissect the relationship between conformational state, lateral mobility, and microclustering of the integrin receptor lymphocyte function-associated antigen 1 (LFA-1) expressed on immune cells. We recently showed that in quiescent monocytes, LFA-1 preorganizes in nanoclusters proximal to nanoscale raft components. We now show that these nanoclusters are primarily mobile on the cell surface with a small (ca. 5%) subset of conformational-active LFA-1 nanoclusters preanchored to the cytoskeleton. Lateral mobility resulted crucial for the formation of microclusters upon ligand binding and for stable adhesion under shear flow. Activation of high-affinity LFA-1 by extracellular Ca(2+) resulted in an eightfold increase on the percentage of immobile nanoclusters and cytoskeleton anchorage. Although having the ability to bind to their ligands, these active nanoclusters failed to support firm adhesion in static and low shear-flow conditions because mobility and clustering capacity were highly compromised. Altogether, our work demonstrates an intricate coupling between conformation and lateral diffusion of LFA-1 and further underscores the crucial role of mobility for the onset of LFA-1 mediated leukocyte adhesion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.