ArticleThe Journal of physiology2012
Activation of glutamate transport evokes rapid glutamine release from perisynaptic astrocytes.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 99 citations in OpenAlex.
- The Role of the Glutamate-Glutamine Cycle in Synaptic Transmission During Ischemia and Recovery.The European journal of neuroscience · 2026Article
- A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis.Journal of neuroinflammation · 2025Article
- Neuropharmacological insight into preventive intervention in posttraumatic epilepsy based on regulating glutamate homeostasis.CNS neuroscience & therapeutics · 2023Review
- Astrocytic modulation of neuronal signalling.Frontiers in network physiology · 2023Review
- The Effects of Modified Curcumin Preparations on Glial Morphology in Aging and Neuroinflammation.Neurochemical research · 2022Review
- Synapse Maturation and Developmental Impairment in the Medial Nucleus of the Trapezoid Body.Frontiers in integrative neuroscience · 2022Review
- Axon-glia interactions in the ascending auditory system.Developmental neurobiology · 2021Review
- Review
- Review
- Activation and Role of Astrocytes in Ischemic Stroke.Frontiers in cellular neuroscience · 2021Review
- The Regulation of Astrocytic Glutamate Transporters in Health and Neurodegenerative Diseases.International journal of molecular sciences · 2020Review
- Auditory brainstem development and plasticity.Current opinion in physiology · 2020Article
- D-Serine Signaling and NMDAR-Mediated Synaptic Plasticity Are Regulated by System A-Type of Glutamine/D-Serine Dual Transporters.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Astrocytes as Guardians of Neuronal Excitability: Mechanisms Underlying Epileptogenesis.Frontiers in neurology · 2020Review
- Sodium Fluctuations in Astroglia and Their Potential Impact on Astrocyte Function.Frontiers in physiology · 2020Review
- Glutamate-Dependent Translational Control of Glutamine Synthetase in Bergmann Glia Cells.Molecular neurobiology · 2018Article
- Glucose and lactate as metabolic constraints on presynaptic transmission at an excitatory synapse.The Journal of physiology · 2018Article
- PKC-Mediated Modulation of Astrocyte SNAT3 Glutamine Transporter Function at Synapses in Situ.International journal of molecular sciences · 2018Article
- Expression of functional inhibitory neurotransmitter transporters GlyT1, GAT-1, and GAT-3 by astrocytes of inferior colliculus and hippocampus.Molecular brain · 2018Article
- Astrocytic Regulation of Glutamate Transmission in Schizophrenia.Frontiers in psychiatry · 2018Review
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4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stimulation of astrocytes by neuronal activity and the subsequent release of neuromodulators is thought to be an important regulator of synaptic communication. In this study we show that astrocytes juxtaposed to the glutamatergic calyx of Held synapse in the rat medial nucleus of the trapezoid body (MNTB) are stimulated by the activation of glutamate transporters and consequently release glutamine on a very rapid timescale. MNTB principal neurones express electrogenic system A glutamine transporters, and were exploited as glutamine sensors in this study. By simultaneous whole-cell voltage clamping astrocytes and neighbouring MNTB neurones in brainstem slices, we show that application of the excitatory amino acid transporter (EAAT) substrate d-aspartate stimulates astrocytes to rapidly release glutamine, which is detected by nearby MNTB neurones. This release is significantly reduced by the toxins L-methionine sulfoximine and fluoroacetate, which reduce glutamine concentrations specifically in glial cells. Similarly, glutamine release was also inhibited by localised inactivation of EAATs in individual astrocytes, using internal DL-threo-β-benzyloxyaspartic acid (TBOA) or dissipating the driving force by modifying the patch-pipette solution. These results demonstrate that astrocytes adjacent to glutamatergic synapses can release glutamine in a temporally precise, controlled manner in response to glial glutamate transporter activation. Since glutamine can be used by neurones as a precursor for glutamate and GABA synthesis, this represents a potential feedback mechanism by which astrocytes can respond to synaptic activation and react in a way that sustains or enhances further communication. This would therefore represent an additional manifestation of the tripartite relationship between synapses and astrocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.