ArticleInvestigative ophthalmology & visual science2012
Nerve growth factor promotes endothelial progenitor cell-mediated angiogenic responses.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Modulation of p75International journal of molecular sciences · 2021Article
- Intranasal Delivery of Nerve Growth Factor in Neurodegenerative Diseases and Neurotrauma.Frontiers in pharmacology · 2021Review
- Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent.Stem cells international · 2021Article
- Antiangiogenic Targets for Glioblastoma Therapy from a Pre-Clinical Approach, Using Nanoformulations.International journal of molecular sciences · 2020Review
- Nerve growth factor regulates endothelial cell survival and pathological retinal angiogenesis.Journal of cellular and molecular medicine · 2019Article
- Xueshuantong Injection (Lyophilized) Attenuates Cerebral Ischemia/Reperfusion Injury by the Activation of Nrf2-VEGF Pathway.Neurochemical research · 2018Article
- Intranasal administration of nerve growth factor promotes angiogenesis via activation of PI3K/Akt signaling following cerebral infarction in rats.American journal of translational research · 2018Article
- Reduced NGF in Gastric Endothelial Cells Is One of the Main Causes of Impaired Angiogenesis in Aging Gastric Mucosa.Cellular and molecular gastroenterology and hepatology · 2018Article
- A Critical Analysis of the AvailableJournal of ophthalmology · 2017Review
- Nerve Growth Factor Promotes Angiogenesis and Skeletal Muscle Fiber Remodeling in a Murine Model of Hindlimb Ischemia.Chinese medical journal · 2016Article
- NGF increases VEGF expression and promotes cell proliferation via ERK1/2 and AKT signaling in Müller cells.Molecular vision · 2016Article
- Silencing p75(NTR) prevents proNGF-induced endothelial cell death and development of acellular capillaries in rat retina.Molecular therapy. Methods & clinical development · 2015Article
- Accelerating in situ endothelialisation of cardiovascular bypass grafts.International journal of molecular sciences · 2014Review
- Biological characteristics of rat dorsal root ganglion cell and human vascular endothelial cell in mono- and co-culture.Molecular biology reports · 2014Article
- Nerve growth factor in diabetic retinopathy: beyond neurons.Expert review of ophthalmology · 2014Article
- Pronerve growth factor induces angiogenesis via activation of TrkA: possible role in proliferative diabetic retinopathy.Journal of diabetes research · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeIn response to ischemia, retinal neuronal cells express nerve growth factor (NGF), which can be proangiogenic. Endothelial progenitor cells (EPCs) can participate with the resident vasculature to promote angiogenesis. We postulated that NGF may stimulate CD34⁺ EPCs to convert to an angiogenic phenotype.
methodsHuman CD34⁺ cells and human retinal endothelial cells (HRECs) were used to examine the effect of NGF on key steps associated with neovascularization. CD34⁺ cells and HRECs were stimulated with NGF (1 to 4 pM) for 24, 48, and 72 hours. Cell migration was measured using a modified Boyden chamber assay. Expression of the receptor for the cytokine stromal derived growth factor 1 (SDF-1), CXCR-4, was assessed by flow cytometry. In vitro angiogenesis was tested using a three-dimensional (3D) extracellular matrix with HRECs/CD34⁺ cell cocultures. NGF receptor activation was assessed by western analysis.
resultsNGF promoted proliferation of CD34⁺ cells but not HRECs. Pretreatment of CD34⁺ cells with NGF increased CXCR-4 expression in CD34⁺ cells, resulting in enhanced migration to SDF-1 (P < 0.0001). The enhanced tubule-forming effect of NGF in HRECs was further potentiated by coculture with NGF-pretreated CD34⁺ cells (P < 0.01). The beneficial effect of NGF was blocked (P < 0.0001) by the ERK inhibitor PD98059. In both CD34⁺ and HRECs, NGF increased phosphorylation of neurotrophic tyrosine kinase receptor type 1 (TrkA) receptor by ERK1 activation (P < 0.01).
conclusionsOur in vitro results suggest that NGF released from ischemic nerves in vivo may contribute to the "angiogenic switch" by stimulating the angiogenic behavior of CD34⁺ cells while minimally affecting resident retinal endothelial cells.
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