ArticleMolecular microbiology2012
Cardiolipin synthase is required for Streptomyces coelicolor morphogenesis.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Involvement of a putative acyltransferase gene in sporangium formation inMicrobiology spectrum · 2024Article
- Omnipresent Maxwell's demons orchestrate information management in living cells.Microbial biotechnology · 2019Article
- Disruption of the GDP-mannose synthesis pathway in Streptomyces coelicolor results in antibiotic hyper-susceptible phenotypes.Microbiology (Reading, England) · 2018Article
- Review
- Cross-membranes orchestrate compartmentalization and morphogenesis in Streptomyces.Nature communications · 2016Article
- Molecular annotation of ketol-acid reductoisomerases from Streptomyces reveals a novel amino acid biosynthesis interlock mediated by enzyme promiscuity.Microbial biotechnology · 2015Article
- Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development.Frontiers in microbiology · 2015Article
- Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.Nucleic acids research · 2013Article
- Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The fluid mosaic model has recently been amended to account for the existence of membrane domains enriched in certain phospholipids. In rod-shaped bacteria, the anionic phospholipid cardiolipin is enriched at the cell poles but its role in the morphogenesis of the filamentous bacterium Streptomyces coelicolor is unknown. It was impossible to delete clsA (cardiolipin synthase; SCO1389) unless complemented by a second copy of clsA elsewhere in the chromosome. When placed under the control of an inducible promoter, clsA expression, phospholipid profile and morphogenesis became inducer dependent. TLC analysis of phospholipid showed altered profiles upon depletion of clsA expression. Analysis of cardiolipin by mass spectrometry showed two distinct cardiolipin envelopes that reflected differences in acyl chain length; the level of the larger cardiolipin envelope was reduced in concert with clsA expression. ClsA-EGFP did not localize to specific locations, but cardiolipin itself showed enrichment at hyphal tips, branch points and anucleate regions. Quantitative analysis of hyphal dimensions showed that the mycelial architecture and the erection of aerial hyphae were affected by the expression of clsA. Overexpression of clsA resulted in weakened hyphal tips, misshaped aerial hyphae and anucleate spores and demonstrates that cardiolipin synthesis is a requirement for morphogenesis in Streptomyces.
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Registered trials
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