Evidence map›Paper›PMID 22408382›Full record

ArticleMolecular breeding : new strategies in plant improvement2012

Diversity arrays technology (DArT) markers in apple for genetic linkage maps.

Henk J Schouten, W Eric van de Weg, Jason Carling, Sabaz Ali Khan, Steven J McKay, Martijn P W van Kaauwen, Alexander H J Wittenberg, Herma J J Koehorst-van Putten, Yolanda Noordijk, Zhongshan Gao and 5 more

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Article in Molecular breeding : new strategies in plant improvement, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

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  19. Highly efficient genotyping of rice biparental populations by GoldenGate assays based on parental resequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Henk J Schouten
W Eric van de Weg
Jason Carling
Sabaz Ali Khan
Steven J McKay
Martijn P W van Kaauwen
Alexander H J Wittenberg
Herma J J Koehorst-van Putten
Yolanda Noordijk
Zhongshan Gao
D Jasper G Rees
Maria M Van Dyk
Damian Jaccoud
Michael J Considine
Andrzej Kilian

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Diversity Arrays Technology (DArT) provides a high-throughput whole-genome genotyping platform for the detection and scoring of hundreds of polymorphic loci without any need for prior sequence information. The work presented here details the development and performance of a DArT genotyping array for apple. This is the first paper on DArT in horticultural trees. Genetic mapping of DArT markers in two mapping populations and their integration with other marker types showed that DArT is a powerful high-throughput method for obtaining accurate and reproducible marker data, despite the low cost per data point. This method appears to be suitable for aligning the genetic maps of different segregating populations. The standard complexity reduction method, based on the methylation-sensitive PstI restriction enzyme, resulted in a high frequency of markers, although there was 52-54% redundancy due to the repeated sampling of highly similar sequences. Sequencing of the marker clones showed that they are significantly enriched for low-copy, genic regions. The genome coverage using the standard method was 55-76%. For improved genome coverage, an alternative complexity reduction method was examined, which resulted in less redundancy and additional segregating markers. The DArT markers proved to be of high quality and were very suitable for genetic mapping at low cost for the apple, providing moderate genome coverage. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-011-9579-5) contains supplementary material, which is available to authorized users.

Identifiers

PMID22408382
PMCPMC3285764

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.