ArticleThe Plant cell2012
The role of a 14-3-3 protein in stomatal opening mediated by PHOT2 in Arabidopsis.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 61 citations in OpenAlex.
- 14-3-3 proteins: Central hubs in plant development and stress adaptation.Journal of integrative plant biology · 2026Review
- TaGF14g, a wheat 14-3-3 protein, acts as a positive regulator of drought and salt tolerance.Plant cell reports · 2025Article
- Mechanistic Insights into the Function of 14-3-3 Proteins as Negative Regulators of Brassinosteroid Signaling in Arabidopsis.Plant & cell physiology · 2024Article
- Article
- Auxin promotes hypocotyl elongation by enhancing BZR1 nuclear accumulation inScience advances · 2023Article
- Light-induced displacement of PLASTID MOVEMENT IMPAIRED1 precedes light-dependent chloroplast movements.Plant physiology · 2022Article
- Light-dependent phosphorylation of THRUMIN1 regulates its association with actin filaments and 14-3-3 proteins.Plant physiology · 2021Article
- Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding.Nature communications · 2021Article
- Reciprocal antagonistic regulation of E3 ligases controls ACC synthase stability and responses to stress.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Ion Homeostasis and Metabolome Analysis ofFrontiers in plant science · 2021Article
- Guard Cells Integrate Light and Temperature Signals to Control Stomatal Aperture.Plant physiology · 2020Article
- Deetiolation Enhances Phototropism by Modulating NON-PHOTOTROPIC HYPOCOTYL3 Phosphorylation Status.Plant physiology · 2019Article
- Light Modulates Ethylene Synthesis, Signaling, and Downstream Transcriptional Networks to Control Plant Development.Frontiers in plant science · 2019Review
- Plant pathogen effector utilizes host susceptibility factor NRL1 to degrade the immune regulator SWAP70.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A chemical genetic approach to engineer phototropin kinases for substrate labeling.The Journal of biological chemistry · 2018Article
- Article
- Regulation of the Regulators: Post-Translational Modifications, Subcellular, and Spatiotemporal Distribution of Plant 14-3-3 Proteins.Frontiers in plant science · 2016Review
- Genome-Wide Identification, Phylogeny, and Expression Analyses of the 14-3-3 Family Reveal Their Involvement in the Development, Ripening, and Abiotic Stress Response in Banana.Frontiers in plant science · 2016Article
- Holophytochrome-Interacting Proteins in Physcomitrella: Putative Actors in Phytochrome Cytoplasmic Signaling.Frontiers in plant science · 2016Article
- New Insights into the Protein Turnover Regulation in Ethylene Biosynthesis.Molecules and cells · 2015Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 14-3-3 λ isoform is required for normal stomatal opening mediated by PHOT2 in Arabidopsis thaliana. Arabidopsis phototropin2 (PHOT2) interacts with the λ-isoform 14-3-3 protein both in yeast two-hybrid screening and in an in vitro pull-down assay. Further yeast two-hybrid analysis also showed that the PHOT2 C-terminal kinase domain was required for the interaction. Site-directed mutagenesis indicated that PHOT2 Ser-747 is essential for the yeast interaction. Phenotypic characterization of a loss-of-function 14-3-3 λ mutant in a phot1 mutant background showed that the 14-3-3 λ protein was necessary for normal PHOT2-mediated blue light-induced stomatal opening. PHOT2 Ser-747 was necessary for complementation of the blue light-activated stomatal response in a phot1 phot2 double mutant. The 14-3-3 λ mutant in the phot1 mutant background allowed normal phototropism and normal chloroplast accumulation and avoidance responses. It also showed normal stomatal opening mediated by PHOT1 in a phot2 mutant background. The 14-3-3 κ mutant had no effect on stomatal opening in response to blue light. Although the 14-3-3 λ mutant had no chloroplast movement phenotype, the 14-3-3 κ mutation caused a weaker avoidance response at an intermediate blue light intensity by altering the balance between the avoidance and accumulation responses. The results highlight the strict specificity of phototropin-mediated signal transduction pathways.
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