ArticleCytoskeleton (Hoboken, N.J.)2012
The microtubule cytoskeleton is required for a G2 cell cycle delay in cancer cells lacking stathmin and p53.
Article in Cytoskeleton (Hoboken, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- MiR-132 controls pancreatic beta cell proliferation and survival through Pten/Akt/Foxo3 signaling.Molecular metabolism · 2020Article
- Tubulin mRNA stability is sensitive to change in microtubule dynamics caused by multiple physiological and toxic cues.PLoS biology · 2019Article
- Reorganization of paclitaxel-stabilized microtubule arrays at mitotic entry: roles of depolymerizing kinesins and severing proteins.Cancer biology & therapy · 2019Article
- The Microtubule Network and Cell Death Are Regulated by an miR-34a/Stathmin 1/βIII-Tubulin Axis.Molecular cancer research : MCR · 2017Article
- Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo.Scientific reports · 2016Article
- Article
- A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Article
- SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status.Cell cycle (Georgetown, Tex.) · 2015Article
- TPX2 is a novel prognostic marker for the growth and metastasis of colon cancer.Journal of translational medicine · 2013Article
- Stathmin and microtubules regulate mitotic entry in HeLa cells by controlling activation of both Aurora kinase A and Plk1.Molecular biology of the cell · 2013Article
- Cardiomyocyte proliferation contributes to heart growth in young humans.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Hypoxia-induced Maspin Expression Affects the Prognosis of Ovarian Clear Cell Carcinoma.In vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In several cancer cell lines, depleting the microtubule (MT)-destabilizing protein stathmin/oncoprotein18 leads to a G2 cell cycle delay and apoptosis. These phenotypes are observed only in synergy with low levels of p53, but the pathway(s) activated by stathmin depletion to delay the cell cycle are unknown. We found that stathmin depletion caused greater MT stability in synergy with loss of p53, measured by the levels of acetylated α-tubulin and the rate of centrosomal MT nucleation. Nocodazole or vinblastine-induced MT depolymerization abrogated the stathmin-depletion induced G2 delay, measured by the percentage of cells staining positive for several markers (TPX2, CDK1 with inhibitory phosphorylation), indicating that MTs are required to lengthen G2. Live cell imaging showed that stathmin depletion increased time in G2 without an impact on the duration of mitosis, indicating that the longer interphase duration is not simply a consequence of a previous slowed mitosis. In contrast, stabilization of MTs with paclitaxel (8 nM) slowed mitosis without lengthening the duration of interphase, demonstrating that increased MT stability alone is not sufficient to delay cells in G2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.