ArticleJournal of assisted reproduction and genetics2012
Development of a novel protocol for isolation and purification of human granulosa cells.
Article in Journal of assisted reproduction and genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Female Age Affects the Mesenchymal Stem Cell Characteristics of Aspirated Follicular Cells in the In Vitro Fertilization Programme.Stem cell reviews and reports · 2019Trial
- Identification of genes differentially expressed in granulosa cells from women with high vs. low ovarian responsiveness.Frontiers in reproductive health · 2026Article
- Screening of Oxidative Stress-related Biomarkers and Antioxidant Drugs for Polycystic Ovary Syndrome Based on Bioinformatics and Molecular Docking.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Current Advances in Cellular Approaches for Pathophysiology and Treatment of Polycystic Ovary Syndrome.Cells · 2023Review
- Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study.Cell journal · 2022Article
- The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Chronic superphysiologic AMH promotes premature luteinization of antral follicles in human ovarian xenografts.Science advances · 2022Article
- Dysregulated sphingolipid metabolism and autophagy in granulosa cells of women with endometriosis.Frontiers in endocrinology · 2022Article
- The Protective Effect of Sulforaphane against Oxidative Stress through Activation of NRF2/ARE Pathway in Human Granulosa Cells.Cell journal · 2021Article
- Does gonadotropin-releasing hormone agonist cause luteolysis by inducing apoptosis of the human granulosa-luteal cells?Journal of assisted reproduction and genetics · 2021Article
- Development of an optimal protocol for isolation and purification of human granulosa cells in patients with different ovarian reserves.Experimental and therapeutic medicine · 2021Article
- Theaflavin 3, 3'-Digallate Delays Ovarian Aging by Improving Oocyte Quality and Regulating Granulosa Cell Function.Oxidative medicine and cellular longevity · 2021Article
- Circular RNA expression profiling of granulosa cells in women of reproductive age with polycystic ovary syndrome.Archives of gynecology and obstetrics · 2019Article
- Article
- Enhanced Inflammatory Transcriptome in the Granulosa Cells of Women With Polycystic Ovarian Syndrome.The Journal of clinical endocrinology and metabolism · 2016Article
- Downregulation of adiponectin system in granulosa cells and low levels of HMW adiponectin in PCOS.Journal of assisted reproduction and genetics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo develop an optimal method of isolation and purification of human granulosa cells from ovarian follicular fluid.
methodsFollicular fluid was collected from patients undergoing oocyte retrieval. A series of isolation and purification techniques was performed, involving density gradient centrifugation and use of different antibody-bead complexes.
resultsThe highest percent yield of live purified granulosa cells came from density gradient centrifugation using sucrose polymer followed by positive selection of granulosa cells using primary antibody to MISRII and secondary antibody coupled to iron oxide beads.
conclusionsA novel protocol for granulosa cell purification has been developed yielding samples that are largely free of nondesirable cells. This protocol provides a purification solution, especially for patient samples that have significant RBC contamination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.