ArticleArchives of virology2012
Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle.
Article in Archives of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- An outbreak of septicemic listeriosis associated with diarrhea in lactating dairy cows.The Journal of veterinary medical science · 2026Article
- Diagnostic Methods for Bovine Coronavirus: A Review of Recent Advancements and Challenges.Viruses · 2025Review
- Detection and characterization of bovine coronavirus and rotavirus in calves in Ethiopia.BMC veterinary research · 2025Article
- Advances in Laboratory Diagnosis of Coronavirus Infections in Cattle.Pathogens (Basel, Switzerland) · 2024Review
- Rotavirus in Calves and Its Zoonotic Importance.Veterinary medicine international · 2021Review
- Bovine coronavirus in neonatal calf diarrhoea in Iran.Veterinary medicine and science · 2020Article
- Longitudinal and cross-sectional detection of four bovine enteric viruses by multiplex- reverse transcription polymerase chain reaction: Identification of possible indicator viruses to assess biosecurity level at bovine farms.The Journal of veterinary medical science · 2020Article
- A novel multiplex PCR-electronic microarray assay for rapid and simultaneous detection of bovine respiratory and enteric pathogens.Journal of virological methods · 2018Article
- Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves.Acta parasitologica · 2017Article
- Molecular detection of enteric viruses from diarrheic calves in Egypt.Archives of virology · 2017Article
- A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay.Journal of virological methods · 2013Article
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9 authors.
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Abstract
A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine coronavirus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 10(2), 10(0), 10(1), and 10(2) TCID(50)/ml, respectively, and that for GBR was 10(6) copies/ml. The one-step multiplex RT-PCR with newly designed primers to detect GAR had higher sensitivity than a single RT-PCR with conventional primers, with no false-positive reactions observed for ten other kinds of bovine RNA viruses To assess its field applicability, 59 of 60 fecal samples containing one of these five viruses from all 25 epidemic diarrhea outbreaks in adult cattle were positive in the one-step multiplex RT-PCR assay. Furthermore, using four additional fecal samples containing two viruses (GBR and BCV or BToV), two amplified products of the expected sizes were obtained simultaneously. In contrast, all 80 fecal samples lacking the five target viruses from normal adult cattle were negative in the multiplex assay. Taken together, our results indicate that the one-step multiplex RT-PCR developed here for the detection of GAR, GBR, GCR, BCV, and BToV can be expected to be a useful tool for the rapid and cost-effective diagnosis and surveillance of viral diarrhea in adult cattle.
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