ArticleNature methods2012
Single-molecule mechanical identification and sequencing.
Article in Nature methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales.Nature protocols · 2024Review
- A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes.Nucleic acids research · 2023Article
- Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding.Communications biology · 2021Article
- Folding and persistence times of intramolecular G-quadruplexes transiently embedded in a DNA duplex.Nucleic acids research · 2021Article
- Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA.Communications biology · 2021Article
- Mechanistic characterization of the DEAD-box RNA helicase Ded1 from yeast as revealed by a novel technique using single-molecule magnetic tweezers.Nucleic acids research · 2019Article
- Multiplex Single-Molecule DNA Barcoding Using an Oligonucleotide Ligation Assay.Biophysical journal · 2018Article
- Unfolding of a ClC chloride transporter retains memory of its evolutionary history.Nature chemical biology · 2018Article
- Asymmetric adhesion of rod-shaped bacteria controls microcolony morphogenesis.Nature communications · 2018Article
- Integrated microHall magnetometer to measure the magnetic properties of nanoparticles.Lab on a chip · 2017Article
- A rapid and practical technique for real-time monitoring of biomolecular interactions using mechanical responses of macromolecules.Scientific reports · 2016Article
- High Spatiotemporal-Resolution Magnetic Tweezers: Calibration and Applications for DNA Dynamics.Biophysical journal · 2015Article
- Extending the range for force calibration in magnetic tweezers.Biophysical journal · 2015Article
- Camera-based three-dimensional real-time particle tracking at kHz rates and Ångström accuracy.Nature communications · 2015Article
- An NGS Workflow Blueprint for DNA Sequencing Data and Its Application in Individualized Molecular Oncology.Cancer informatics · 2015Review
- Plant exomics: concepts, applications and methodologies in crop improvement.Plant signaling & behavior · 2015Review
- Stochastic ratchet mechanisms for replacement of proteins bound to DNA.Physical review letters · 2014Article
- Reconstruction and identification of DNA sequence landscapes from unzipping experiments at equilibrium.Biophysical journal · 2014Article
- The evolution of nanopore sequencing.Frontiers in genetics · 2014Review
- DNA polymerases drive DNA sequencing-by-synthesis technologies: both past and present.Frontiers in microbiology · 2014Review
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Authors and funding
7 authors.
Funding
Abstract
High-throughput, low-cost DNA sequencing has emerged as one of the challenges of the postgenomic era. Here we present the proof of concept for a single-molecule platform that allows DNA identification and sequencing. In contrast to most present methods, our scheme is not based on the detection of the fluorescent nucleotides but on DNA hairpin length. By pulling on magnetic beads tethered by a DNA hairpin to the surface, the molecule can be unzipped. In this open state it can hybridize with complementary oligonucleotides, which transiently block the hairpin rezipping when the pulling force is reduced. By measuring from the surface to the bead of a blocked hairpin, one can determine the position of the hybrid along the molecule with nearly single-base precision. Our approach can be used to identify a DNA fragment of known sequence in a mix of various fragments and to sequence an unknown DNA fragment by hybridization or ligation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.